Bulletin "Veterinary biotechnology"

Veterynarna biotehnologija – Veterinary biotechnology, 2018, 32, 537-545 [in Ukrainian]. https://doi.org/10.31073/vet_biotech32(2)-65

TARASOV O.A., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., SAPEYKO V.P., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., BABKINA M.M., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., TERESCHENKO S.M., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., KRILENKO S.U., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., GUMENIUK V.V., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

Institute of Veterinary Medicine of NAAS

SHEVCHENKO T.V., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

National Academy of Agrarian Sciences of Ukraine


Introduction. Nowadays the important task for scientists is to intensify search for new compounds with antimicrobial activity of different chemical classes what can be used as a base for creating new antimicrobial preparations.

The goal of the work was to study antibacterial properties of experimental preparation against test microorganisms.

Materials and methods. In this work it was used the substances of fluorequinolones class (enrofloxacin, norfloxacin, ofloxacin, cyprofloxacin) and microorganisms: Staphylococcus aureus Р209, Micrococcus flavus ATCC10240, E. coli 1257, Proteus vulgaris, Clostridium perfringens, Erysipelothrix rhusiopathiae strain К, Erysipelothrix rhusiopathiae М-2 ВК, Micrococcus luteus ATCC 9341, Bacillus subtilis ATCC663 and pathogenic field isolates were used. In the research the disc-diffusion and method of serial microdilution were used. The quantity of susceptible strains and field isolates against tested antibiotics was assessed.

Results of research and the discussion. During the laboratory testing of our experimental composition with enrofloxacin (5%) and sulphadimethoxine (20%), a high antibacterial activity against all tested strains of microorganisms was revealed, the MIC varied from 0.20±0.12 mcg/cm3 with E. coli 1257 to 0.4±0.10 mcg/cm3 with Clostridium perfringens. These data corresponded to the literature data. The MIC of the experimental composition was the lowest among all tested substances with Staphylococcus aureus P209 (0.80±0.05 mcg/cm3), Proteus vulgaris (0.20±0.15 mcg/cm3), Clostridium perfringens (0.32±0.10 mcg/cm3). Analysis of the antibiotic sensitivity showed that the total number of pathogenic strains of E. coli sensitive to the experimental preparation was 77.7%. The experimental composition showed the highest possible efficacy against Salmonella choleraesuis (100%) and Staphilococcus aureus (100%), followed by E. coli (77.7%), Pasteurella multocida (75.0%) and Clostridium perfringens (72.7%). The moderate level of sensitivity was detected for Streptococcus zooepidemicus (56.2%). Pathogenic E. coli were resistant in vitro in 4 cases (23.3%), Streptococcus zooepidemicus – in 5 cases (43.8%), Pasteurella multocida – in 3 cases (25%) and Clostridium perfringens – in 3 cases (27.3% ) and in one case – to Klebsiella spp. (11.2%).

Conclusion and prospects for further research. When studying the MIC and the growth inhibition with a disc-diffusion method, we found that all the studied strains were highly sensitive to both enrofloxacin and to the experimental composition. The data obtained reveal some trends in the development of the microflora resistance to the preparation components, what may be explained due to the continuous using of fluoroquinolone antibiotics in the farms where tested microorganisms were isolated.

Keywords: antibiotic preparation, antimicrobial action, fluorequinolones.


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