Veterynarna biotehnologija – Veterinary biotechnology, 2018, 32, 46-51 [in Ukrainian]. https://doi.org/10.31073/vet_biotech32(2)-04
Odessa Research Station of the National Science Center “IECVM”
Odessa State Agrarian University
BIOCHEMICAL INDICATORS OF BLOOD SERUM OF CALVES DURING MIXED PASSING OF CRYPTOSPORIDIOSIS AND EIMERIOSIS
Introduction. Parasite cenosis is characterized by the presence in the macroorganism of several parasitic agents of different taxonomic groups and can cause the emergence of associative diseases. Cryptosporidia is often parasitized in combination with the eimeria and helminthes, which leads to complications of healing and improving measures and rising of death level of young animals.
The goal of the work. Find out the influence of cryptosporidia and eimeria on the biochemical parameters of blood serum of calves of 35 days old – the time of diet change to coarse forage.
Materials and methods. Parasitic, biochemical, immunological, statistical.
Results of research and discussion. In the experimental group of animals the content of total protein was reduced by 9.3% and albumin content by 26.2%. Such changes in the blood serum of sick calves indicate on derangement of digestion and absorption of the products of hydrolysis of proteins in the intestine. Total globulins increased by 8.9%. The number of α-globulins did not change significantly, while β-globulins increased by 21.2% and γ-globulins by 8.8%. The ratio of albumins to globulins formed an appropriate coefficient of 0.7 in the experimental group versus 1.1 in the control. In infected calves an increase in the activity of transaminases (AAT) was observed by 11.4% and ALT by 31%, which indicates on the presence of degenerative processes in the liver. The concentration of circulating immune complexes in the blood serum of calves in the experimental group was 76.49±2.05 mg/cm3, which was by 23.5% higher than in the control group 61.92±1.01 mg/cm3. This indicates the development of immunosuppression in the animal's body after invasion. With the development of the invasion, a probable increase of seromucoid concentration by 35.7% and amounted 0.19±0.02 mg/cm3 against 0.14±0.01 mg/cm3 in the control, which indicating the presence of mechanical damage of the tissues.
Conclusions and prospects for further research. In the biochemical composition of blood serum of calves, spontaneously infected with cryptosporidia and eimeria, the decrease in the content of total protein is associated with a violation of the protein synthesis function of the liver due to intoxication of the animal's organism with the products of metabolism of parasites. Reducing of the content of albumin indicates a liver dysfunction, and an increase in β-globulins indicates an inhibition of the immune response to the invasion. Increasing of the activity of the enzyme AAT indicates on the development of degenerative processes in the liver, which plays a primary role in the neutralization of toxins that are formed in the body of animals during the invasion.
Keywords: cryptosporidiosis, eimeriosis, calves, blood.