Bulletin "Veterinary biotechnology"

Veterynarna biotehnologija – Veterinary biotechnology, 2018, 32, 72-76 [in Ukrainian]. https://doi.org/10.31073/vet_biotech32(2)-08

GALAT M.V., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., KOVALENKO G.A.1, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., GALAT V.F., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., HALKA I.V.1, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., SYTIUK M.P.1, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., NYCHYK S.A.1, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

National University of Life and Environmental Sciences of Ukraine

1 Institute of Veterinary Medicine of the NAAS

PREVALENCE OF TOXOPLASMOSIS AMOUNG WILD BOARS IN THE TERRITORY OF UKRAINE

Introduction. The article presents the results of research of wild boar (Sus scrofa) blood serum for the presence of antibodies of toxoplasmosis agent Toxoplasma gondii. The data obtained in the course of an experimental study is based on the spreading of toxoplasmosis among wild boars from different regions of Ukraine. At the next stage, a comparison of the obtained data with the similar results of serological investigation of pigs (Sus scrofa domesticus) of different age groups, breeds and conditions of containment of some regions of Ukraine was made.

The goal of the work. To study the prevalence of toxoplasmosis among wild boars in Ukraine. Compare the obtained results with the extensiveness of the infection among pigs under different conditions of retention.

Materials and methods. The research was conducted during 2013–2018 on the basis of the Scientific-Research Training Center for Animal Disease Diagnostics at the Institute of Veterinary Medicine of the National Academy of Agrarian Sciences of Ukraine, on the Department of Parasitology and Tropical Veterinary Medicine and the Ukrainian Laboratory of Quality and Safety of APC at the National University of Life and Environmental Sciences of Ukraine. For this purpose, 360 samples of wild boar blood from 16 regions of Ukraine and 169 samples from pigs of various breeds and age groups of farms with different forms of ownership on the territory of Poltava, Lviv, Cherkasy and Khmelnytsky regions were used. The serological studies of these samples were performed using the immunoassay method with the help of the commercial test kit VektoToxo-antibodies (VektorBest, Russian Federation) in accordance with the manufacturer's instructions.

Results of research and discussion. According to the results of the first investigation in Ukraine, 24 positive samples of blood serums of wild boar (Sus scrofa) from the examined 360 were found (6.67%). According to preliminary studies of pig blood serum (Sus scrofa domesticus), 28 animals from the examined 169 or 16.57% were positive to the agent.

Conclusions and prospects for further research. Since the meat of wild boar meat is one of the sources of toxoplasmosis infection of different species of animals and humans, the issue of the spread of the T. gondii agent in the territory of our country among the wild fauna is of considerable interest. Such studies were conducted in the territory of Ukraine for the first time and they require to be continued. In our next investigations we planned to conduct molecular genetic studies to confirm the results of the serological investigations.

Keywords: toxoplasmosis; Тoxoplasma gondii; serological diagnostics; wild boar; Sus scrofa; Sus scrofa domesticus.

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