Bulletin "Veterinary biotechnology"

Veterynarna biotehnologija – Veterinary biotechnology, 2018, 32, 100-112 [in Ukrainian]. https://doi.org/10.31073/vet_biotech32(2)-12

GORBATIUK O.I., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., MINTSIUK E.P., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., ANDRIYASCHUK V.A., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., RYZHENKO G.F., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., ZHOVNIR A.M., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., REZNICHENKO L.S.1, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., DYBKOVA S.N.1, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., UKHOVSKA T.N., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., TIUTIUN S.N., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., KRYLENKO S.YU., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

Institute of Veterinary Medicine of the NAAS

1 F. D. Ovcharenko Institute of Biocolloidal Chemistry

THE STUDY OF THE MEMBRANE ATPase ACTIVITY LEVEL OF C. PERFRINGENS AND GOLD NANOPARTICLES’ EFFECT CRITERIA ON ITS METABOLIC PROCESSES

Introduction. In the biotechnology of veterinary immunobiological means, it is important to obtain specific highly concentrated antigens. Membrane H+-ATPase is one of the main generators of the transmembrane potential of bacterial cells; therefore, the level of its activity can predict the criteria for the nanopreparations effect on the metabolic processes of anaerobic bacterial cells.

The goal of the work was to study the degree of dependence between the of C. perfringens types A, B, C, D membrane activity after gold nanoparticles application and the criteria of their influence on the growth and multiplication of bacteria with the determination of stimulating concentrations to improve the growth qualities of the medium.

Materials and methods. The metabolic processes of C. perfringens types A, B, C, D were investigated. We used bacteriological, biochemical, statistical methods of research.

Results of research and discussion. The results of the studies showed decreasing in the activity of the C. perfringens type A membrane enzyme after the gold nanoparticles application in the range of 38.6 to 0.386 μg/ml by metal, which negatively affected the metabolic processes of the pathogen cells, which confirmed their correlation. Stimulation of C. perfringens type A metabolic processes was observed in the range of 0.15 μg/ml by metal and lower. The activity of the C. perfringens type B membrane fraction after the application of gold nanoparticles increased significantly by 20.1% compared to the control. The activation of H+-ATPase confirmed the positive effect of gold nanoparticles on the growth and multiplication of this strain. The dependence of the ATPase membrane activity level and metabolic processes of C. perfringens type C was confirmed by the results of studies, since the gold nanoparticles effect, in the range from 38.6 to 0.386 μg/ml by the metal, on the pathogen growth and multiplication was tolerant in all cases. The indices of the H+-ATPase activity of C. perfringens type D level under the gold nanoparticles effect in the range from 38.6 to 3.86 μg/ml by the metal and the metabolic processes were slightly suppressed or tolerated. The gold nanoparticles in concentration from 0.386 μg/ml by the metal effected the growth of the ATPase activity level as well as growth and multiplication of the pathogen cells.

Conclusions and prospects for further research. The dependence between the H+-ATPase activity of the C. perfringens types A, B, C, D membrane fractions and the pathogen metabolic processes character under the gold nanoparticles effect was found. The technological method for the application of stimulating concentrations of gold nanoparticles for C. perfringens type A – 0.038; type B – 0.009; type C – 0.0047 and type D – 0.30 μg/ml by the metal was developed to obtain highly concentrated specific antigens. Prospects for further research include studies on the gold nanoparticles effect on the cellular and humoral immunity.

Keywords: membrane H+-ATPase, gold nanoparticles, C. perfringens types А, В, С, D; inhibitory effect, stimulation.

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