Veterynarna biotehnologija – Veterinary biotechnology, 2016, 28, 13-20 [in Ukrainian].

AYSHPUR O.Y., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., SAPON N.V., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., MUSHTUK I.Y., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., ZOZENKO I.A., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., SHEREMET N.O., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

Institute of Veterinary Medicine of NAAS

SENSITIVITY OF BACTERIA OF GENUS PROTEUS TO ANTIBACTERIAL PREPARATIONS

Introduction. In recent years, the attention of infectionists focused on so-called "new" infections, which are caused by nonconventional microorganisms among which a special place is occupied by proteus infection.The results of research on the study of Proteus biology in recent years suggest etiologic role of these microorganisms in the course of various inflammatory and purulent processes. In addition, Proteus bacteria form association with pathogenic and opportunistic bacteria, increasing their negative effect on the macroorganism. Most authors considering that Proteus against increases the toxic properties of infection pathogens, reduces performance leukocytes phagocytosis indices delaying the action of antibiotics or increases resistance of associated bacteria to them, but the mechanisms of these effects have not been studied. Proteus genus bacteria affects the digestive tract, and respiratory, hearing, organs,urinary tract, nervous system and others.

The goal of the work. To conduct a study of Proteus bacteria sensitivity to antimicrobial preparations, isolated from biomaterials of a live and dead animals.

Materials and methods of research. The study was conducted in laboratory of the bacterial animals diseases and control of VIP of the Institute of Veterinary Medicine of the National Academy of Agrarian Sciences of Ukraine. The bacterial cultures of Proteus genus, isolated from biomaterials of alive and dead animals were tested. The morphological, cultural, biochemical and pathogenic properties of isolated cultures of microorganisms were determined by conventional methods in microbiology using conventional and selective growth media.

Results of research and discussion. Our results indicate that of Proteus bacteria involved in inflammatory processes at different course of diseases in different species of productive animals, which complicates the course of disease and causes considerable damage to livestock.The data indicate variability of isolated cultures resistance against antibiotics. Analyzing the results, we can conclude that the Proteus more susceptible to aminoglycoside, cephalosporins, glycopeptides, polypeptides, macrolides; less sensitive to penicillin, sulfonamides, insensitive to lincomycin and resistant to tiamulinum. According to the research results were found microbial cultures that have been completely susceptible to all antibiotics defined group. Therefore, therapeutic drugs for any inflammation in the animals organism must be used according to data on antibiotic resistance and use comprehensive treatment.

Conclusions and prospects of further research: 1. According to our research Proteus bacteria are involved in inflammatory processes at different symptom and diseases in different species of productive animals, including: mastitis, endometritis, hoof rot in cows; respiratory syndrome in calves; the complex of respiratory and gastrointestinal diseases of pigs; at endometritis and aborts of unexplained etiology in sows; at purulent pneumonia in rabbits. 2. Proteus bacteria are more sensitive to aminoglycoside (37.5–100% isolates), cephalosporins (66.0–100%), glycopeptides (57.1–80.0%), polypeptides (20.0–88.9%) macrophytes (33.3–45.5%); less susceptible to penicillin (0.0–42.9%), sulfonamides (0.0–20.0%), insensitive to lincomycin (20.0–25.0%) and resistant to tiamulinum indicating changes of resistance. 3. Given that cultures of microorganisms were found that would have been completely susceptible to antibiotics of certain groups of medicines for any inflammation in the animal organism must be used according to data on antibiotic resistance, and in complex, depending on their compatibility. The phenomenon of antimicrobial resistance of Proteus bacteria and what determines this property is need of further research.

Keywords: bacteria, genus Proteus, isolates, resistance, sensitivity, antibiotics.

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