Bulletin "Veterinary biotechnology"

Veterynarna biotehnologija – Veterinary biotechnology, 2016, 28, 28-41 [in Ukrainian].

GARKAVENKO T.A., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., AZYRKINA I.M, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., KYIVSKA G.V., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

State Research Institute of Laboratory Diagnostics and Veterinary and Sanitary Expertise


Introduction. One of the main indicators characterizing the safety of poultry products is the absence or presence in concentrations residues of antimicrobial drugs below the permissible limits. Methods of inhibiting antimicrobial agents are recognized as the best research methods. This method is quite objective, based on the standard strains of microorganisms susceptibility to antibiotics.

The goal of the work was the comparative analysis of the national and European microbiological screening methods for antibiotic residual in poultry meat and eggs.

Results of research and discussion. Since September 2016 in connection with the entry into force of the Order "On approval of safety parameters of poultry" from 06.08.2013. N695 criteria of studying the poultry products under the periodic control of antibiotics traces are expanded. Goal is to develop and implement to the laboratory practice sensitive, simple, cheap, readily available screening methods, such as microbiological method. «A new microbial screening method for the detection of antimicrobial residues in slaughter animals: The Nouws antibiotic test (NAT-screening)»; was validated and approved in accordance with the 2002/657/EC and accredited by the Dutch Accreditation Council in ISO 17025. Since March 2004 method research under state monitoring plan according to Council Directive 96/23/EC. The sensitivity of this method corresponds EU MRL normative documents and makes it possible to determine the antibiotic group and 6 sulfanilamidnye drugs. This five cup method based on the principle of agar diffusion of antimicrobial agents. Method allows you to explore a large number of samples and require minimum amount of time and consumables, provides the identification of residual amounts of antimicrobial agents to the group.

Conclusions and prospects for further research: 1. Today in Ukraine using microbiological method in poultry products we can determine only residual amounts of tetracycline antibiotics group zinc bacitracin, streptomycin. 2. The method of «NAT-screening» provides an opportunity to explore the remaining number of 43 antibiotics that belong to six groups and sulfanilamides.

Keywords: Preparations antimicrobial, microbial screening method, poultry meat, eggs, NAT-screening, test microorganisms culture.


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