Veterynarna biotehnologija – Veterinary biotechnology, 2016, 28, 84-93 [in Ukrainian].
Sumy National Agrarian University
Poltava State Agrarian Academy
State Research Institute of Laboratory Diagnostics and Veterinary and Sanitary Expertise
COMPARATIVE PATHOMORPHOLOGICAL EVALUATION MANIFESTATION OF ASPERGILLOSIS' MANIFESTATION CAUSED BY ASPERGILLUS FLAVUS IN DIFFERENT SPECIES OF POULTRY
Introduction. Aspergillosis is a disease of birds caused by fungi genus Aspergillus and characterized by lesions of the respiratory and serous membranes. In the literature pathological-anatomical changes of aspergillosis in birds are caused by Aspergillus flavus and described not enough. In addition, the lack of reports of histopathological changes in disease caused by A. flavus, in such birds like ducks, swans, ostriches and other.
The goal of the work. It was to study the pathological-anatomical and pathological-histological manifestation of aspergillosis in different species in poultry caused by A.flavus.
Materials and methods. A pathological-anatomical and histopathological study of pathological material obtained from different species of birds (swans, geese and ostriches), which died from aspergillosis. Histopathological study included alcohol wiring pathological material, filled in paraffin and staining with hematoxylin-eosin. As laboratory diagnostic methods it was used mycological research that included hanging on Sabourauds dexrose agar, microscopy and identification of A. flavus.
Results of research and discussion. It was found that swan fibrinous layering was observed in serous membranes and respiratory bags, cheesy nodules in lungs. In ducks revealed multiple nodules, plaques in the wall of pneumatic bags, venous congestion and pulmonary edema, signs of liver and catarrhal enteritis. It was observed numerous granulomas in lungs and respiratory bags, catarrhal, fibrinous rhinitis and laryngitis in young ostriches. Formation of fungal granulomas had a similar trend and was characterized by the emergence pseudoeosinophiles at first, which later had been changed on epithelioid, lymphoid and some giant cells on the periphery of granulomas that are elements of connective tissue capsule.
Conclusions and prospects for further research: 1. The peculiarities of pathological-anatomical manifestations in cases of aspergillosis caused by A. flavus, were found in various species of birds. 2. In a swan with aspergillosis were found fibrinous layers on serous membranes and respiratory sacks in the form of films and cheesy nodules in the lungs. In ducks multiple nodules, plaques in the wall of pneumatic bags, venous congestion and pulmonary edema, signs of liver and catarrhal enteritis were observed. In ostriches aspergillosis was manifested by numerous nodules in lungs and respiratory bags, catarrhal, fibrinous rhinitis and laryngitis. 3. The formation of fungal granulomas occurred a classic case: originally appeared pseudo- eosinophiles that later on were substituted with epithelioid, lymphoid and some giant cells. On the periphery items it was found granulomas formation of connective tissue capsule. 4. Identification of inflammation in the small intestine and degenerative processes in the liver and kidneys may indicate a toxic effect of metabolic products of Aspergillus flavus on organism of birds. For the further research it is planned to find out the possibility of defeat on tissue level of other organs and systems in different types of poultry aspergillosis caused by A. flavus.
Keywords: Aspergillus flavus, poultry, histopathological studies, mycological studies, aspergillosis.