Veterynarna biotehnologija – Veterinary biotechnology, 2016, 28, 108-114 [in Ukrainian].

KOVALENKO G.A., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., HALKA I.V., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., RUDOJ A.V., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., SIDORENKO T.V., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., MUZYKINA L.N., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., POGREBNJAK O.P., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

Institute of Veterinary Medicine of NAAS

A SEROLOGICAL SURVEY OF MYCOPLASMOSIS (M. HYOPNEUMONIAE) AMONG WILD BOARS IN NORTH-WESTERN OBLASTS OF UKRAINE

Introduction. Mycoplasmosis – porcine enzootic pneumonia is a chronic infectious disease that increases the susceptibility of pigs to secondary infections, which makes the disease course heavier, often resulting in death.

The goal of the work. To determine the seroprevalence of enzootic pneumonia in wild boars in north-western regions of Ukraine.

Materials and methods. Serum of wild boars were obtained from 6 oblasts of Ukraine: Volyn, Rivne, Zhytomyr, Kyiv, Chernihiv and Vinnitsia. 120 samples were investigated. Indirect ELISA assay was used for detecting serum antibodies to Mycoplasma hyopneumoniae using a commercial Test Kit «M. hyo. Ab» IDEXX, USA.

Results of research and discussion. Antibodies to M. hyopneumoniae were detected in 70.0%, 30.0% and 85.0% of sera samples from Volyn, Rivne and Zhytomyr oblasts, respectively. From Kiev and Chernihiv oblasts the percentage of seropositive samples was 65.0% and 55.0%, respectively. We found antibodies to M. hyopneumoniae in 2 (10.0%) of 20 blood serum samples of wild boars from Vinnitsa oblast. According to the research the highest percentage of seropositive samples detected in wild boars from Zhytomyr, Volyn and Kyiv oblast. The smallest percentage of seropositive samples was found in Vinnytsia and Rivne oblast. The total number of seroprevalence of wild boars to M. hyopneumoniae in all listed oblasts of Ukraine was 52.5% of all total investigated sera. The total rate of negative and doubtful samples was 40.0% and 7.5% respectively. Results are expressed as S/P values (sample to positive control ratio) according to the manual of the test kit. The result was considered positive if the S/P of the sample in the well was exceeded the value of 0.40. The highest S/P values were found in the positive sera samples of wild boars from Zhytomyr oblast. The average S/P value was 1.31±0.11. The average S/P value of positive sera samples of wild boars from Vinnitsa oblast was 0.42±0.01, that is on the verge of the threshold values. Strong performances of S/P of positive samples were also determined in wild boars from Kyiv and Chernihiv oblasts. Average S/P value was 0.93±0.11 and 0.91±0.07 respectively.

Conclusions and prospects for further research. The presence of antibodies to M. hyopneumoniae in the blood sera of wild boars confirms the circulation of the pathogen in wild fauna in Ukraine. Serological survey data indicate that a contact of wild boars with the pathogen in different oblasts of Ukraine significantly correlated. Thus, the findings point out the necessity of further continuous monitoring of enzootic pneumonia in swine farms in Ukraine.

Keywords: M. hyopneumoniae, ELISA, antibody, seroprevalence, wild boars, Ukraine.

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