Veterynarna biotehnologija – Veterinary biotechnology, 2016, 28, 149-158 [in Ukrainian].
Institute of Veterinary Medicineof NAAS
GENETIC DIVERSITY OF RABIES VIRUS AND OTHER LYSSAVIRUS OF ANIMALS
Introduction. Rabies infection is characterized by lesion of central nervous system (signs of encephalomyelitis). Each year over 60,000 people and more than 1 million animals die worldwide, and more than 20 million people about 40% of which are children under 15 years receive rabies vaccination after bites. WHO Expert Committee on Rabies in its recommendations emphasizes the need to conduct typing of street isolates rabies virus based on virological and molecular genetic techniques.
The goal of the work. To analyze statistics and literature data, to study the diversity of Lyssaviruses genotypes circulating in the wildlife.
Materials and methods. For the studies, we used scientific papers, monographs, abstracts of dissertations, electronic internet resources.
Results of research and discussion.Bats are reservoirs and vectors for six of the seven classified genotypes. For five genotypes (LBV, DUVV, EBLV type 1, ABLV type 2, ABLV) bats are the only vectors. Only MOKV was not isolated from bats. Not classified genotypes of rabies virus and rabies-like Lyssaviruses also isolated from bats in various continents. Every year in the European countries it is recorded a hundred cases of rabies in bats. It was registered 5 cases of rabies in humans after being bitten by bats including three on the southeast of Ukraine and adjacent territory of the Russian Federation. In Ukraine from 2000 to 2015 it was laboratory confirmed 16 cases of rabies-like infection in bats but all the causative agents have never been identified.
Conclusions and prospects for further research:
- Lyssavirus is found in bat populations throughout the world.
- A wide range of antigenic and genetic Lissaviruses requires improvement and development of highly immunogenic vaccines against circulating genotypes.
- In Ukraine in 1977, 1985, and 2002 there were recorded three cases of hydrophobia in humans after unprovoked attack of bats and only in one case the virus was identified as EBLV-1.
- The perspective is the study of selected viral isolates, identification and typing through sequencing and phylogenetic analysis, and then comparing the results with already known genotypes of rabies virus circulating in the wild.
Keywords: rabies, bats, genotypes, lyssavirus.
- WHO Expert Consultation on Rabies. (2005). Technical Report Series 931. Geneva: World Health Organization.
- WHO Expert Consultation on Rabies (2013). Technical Report Series 982. Geneva: World Health Organization.
- Fauquet, C.M., Mayo, M., & Maniloff, A.J. (2005). Virus taxonomy. Classification and Nomenclature of Viruses. Eighth report of the International Commitee on the Taxonomy of Viruses. Virology Division International Union of Microbiological Societies.
- Fooks A., McElhinney L., & Pounder D. (2003). Isolation of a Europen bat lyssavirus type-2 a from fatal human case of rabies encephalitis. Case report. Medical Virology, 71, 281-289.
- Gould, A.R., Hyatt, A.D., & Lunt, R. (1998). Characterization of a novel lyssavirus isolated from Pteropid bats in Australia. Virus Research, 54, 165-187.
- Kaplan, M., & Koprowski, H. (1996). Laboratory techniques in rabies. Geneva: World Health Organization, (Vol. 4).
- International Committee on Taxonomy of Viruses. Retrieved from http://www.ictvonline.org/virusTaxonomy.asp.
- Tordo, N., & Poch, O. (1998). Structure of rabies virus. Kluwer Academic Publishers. Boston. 25-45.
- Nadin-Davis, S.A. (2000). Rabies and rabies-related viruses. Oxford University Press. Molecular Epidemiology of Infectious Diseases. New York. 245-257.
- Rudd, R., Smith, J., & Yager, P. (2005). A need for standardized rabies-virus diagnostic procedures: effect of cover-glass mountant on the reliability of antigen detection by the fluorescent antibody test. Virus Research, 111, 83-88.
- Bourhy, H., Kissi, B., & Tordo, N. (1993). Molecular Diversity of the Lyssavirus Genus Virology, 194, 70-81.
- Bourhy, H., Kissi, B., & Tordo, N. (1995). Molecular epidemiological tools and phylogenetic analysis of bacteria and viruses with special emphasis on lyssaviruses. Preventative Veterinary Medicine, 25, 161-181.
- Africa Bourhy, H., Kissi, B., & Tordo, N. (1993). Taxonomy and evolutionary studies on Lyssaviruses with special reference to Onderstepoort. Journal of Veterinary Science, 60, 277-282.
- Domingo, E. (1997). Rapid Evolution of Viral RNA Genomes. Journal of Nutrition, 127, 958-961.
- Kuzmin, I.V., Hughes, G.J., & Botvinkin, A.D. (2005). Phylogenetic relationships of Irkut and West Caucasian bat viruses within the Lyssavirus genus and suggested quantitative criteria based on the N gene sequence for lyssavirus genotype definition. Virus Research, 111, 28-43.
- Kuzmin, I.V., Niezgoda, M., Franka, R., Agwanda, B., Markotter, W., & Beagley, J.C. (2008). Possible emergence of West Caucasian bat virus in Africa. Emerging Infectious Diseases, 14, 12, 1887-1889.
- Kuzmin, I.V., Niezgoda, M., Franka, R., Agwanda, B., Markotter, W., & Beagley I. (2008). Lagos bat virus in Kenya . Clinical Microbiology, 46, 4, 1451-1461.
- Kuzmin, I.V., Orciari, L.A., Arai, Y.T., Smith, J.S., Hanlon, C.A., & Kameoka, Y. (2003). Bat lyssaviruses (Aravan and Khujand) from Central Asia: phylogenetic relationships according to N, P and G gene sequences. Virus Research, 97, 65-79.
- Kuzmin, I.V, Anne E. Mayer, & Niezgoda Michael. (2010). Shimoni bat virus, a new representative of the Lyssavirus genus. Virus Research, 149, 197-210.
- Kuzmin I.V, Wu X.F., Tordo N., & Rupprecht C.E. (2008). Complete genomes of Aravan, Khujand, Irktu and West Caucasion bat viruses, with special attention to the polymerase gene and non-coding regions. Virus Research, 136, 81-90.
- Rabies-Bulletin-Europe. Information Surveillance Report. Retrieved from http://www.who-rabies-bulletin.org/Queries/Dynamic.aspx
- Selimov, M.A., Smekhov, A.M., & Antonova, L.A. (1991). New strain of rabies-reletad viruses isolated from bats in the Ukraine. Acta Virologica, 35, 226-231.
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