Bulletin "Veterinary biotechnology"

Veterynarna biotehnologija – Veterinary biotechnology, 2016, 28, 181-187 [in Ukrainian].

MANOILO Y.B., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., YEVSTAFIEVA V.A., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

Poltava State Agrarian Academy

EFFECTIVENESS OF THE IMPROVED METHOD OF COPRO-OVOSCOPIC DIAGNOSTICS OF OESOPHAGOSTOMIASIS IN PIGS

Introduction. The diagnostics of pig nematoses (including pig oesophagostomiasis) has some peculiarities and is the main stage in the prevention and treatment of infections.

According to many authors, copro-ovoscopic methods of pig nematoses diagnostics are characterized by varying diagnostic effectiveness depending on specific weight, viscosity, degree of solution crystallization, time of rising of maximum egg number on the surface of flotation solution. Some of the methods are laborious in practice and require time, financial expenses or are simply not efficient enough.

The goal of the work was to propose a method of intravial diagnostics of pig oesophagostomiasis.

Materials and methods. 50 fecal samples of pigs with oesophagostomiasis were studied according to V.N. Trach’s method. The infected material was studied using three traditional methods (Kotelnikov-Khrenov, Fulleborn, and Mallory), and according to the improved experimental one. In total, 600 studies were made.

Results and discussion. The effectiveness of improved copro-ovoscopic diagnostic method of pig oesophagostomiasis is proved to be high (р<0.001) compared to the generally known methods. The Fulleborn copro-ovoscopic method allowed estimating the pig oesophagostomiasis intensity from 21.28±1.7 up to 26.96±1.2 EGF depending on exposition time. Counts of nematode eggs using this method were difficult because of moderate amounts of feed remains that also seen in the field of microscope.

The effectiveness of Kotelnikov-Khrenov method used for pig oesophagostomiasis diagnostic was higher by 25.6–35.8 % than that Fulleborn’s. There was a lot of fecal matter in the field of microscope, which was dirtying the surface film of samples, thus making the detection of Oesophagostomum eggs difficult.

The Mallory coproscopic method allowed estimate of Oesophagostomum infection ranging from 29.6±1.8 to 40.16±1.3 EGF and was more effective by 14.2–38.8% than Fulleborn’s one. At the same time, effectiveness of Mallory method was lower by 2.1–25.1% than that of Kotelnikov-Khrenov one. Diagnostics using this method was difficult because of moderate amounts of feed remains that also seen in the field of microscope.

The most effective for copro-ovoscopic diagnostics of pig oesophagostomiasis was the improved method when using saturated solution of sugar and sodium chloride (1 : 1) as flotation solution. The proposed flotation solution has higher ability of coagulation of undigested food remains which greatly facilitates microscopic studies and enhances the effectiveness of detection of helminth eggs.

Conclusions and prospects for future research.

  1. The proposed method of diagnostics of pig oesophagostomiasis is proved to be highly effective by 49.4%, 29.7% and 28.1% (р<0.001) than the Fulleborn, Kotelnikov-Khrenov and Mallory methods respectively.
  2. Intravial improved method of pig oesophagostomiasis diagnostics provides high definition of studied material and ensures zero contamination with associated organic material.
  3. Optimal time for high diagnostic effectiveness of the improved method is ten minutes.

Keywords: pigs, oesophagostomiasis, intravial diagnostics, copro-ovoscopy, microscopy.

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