Veterynarna biotehnologija – Veterinary biotechnology, 2016, 28, 232-241 [in Ukrainian].
Institute of Veterinary Medicineof NAAS
National University of Life and Environmental Sciences of Ukraine
PATOMORPHOLOGYCAL RESEARCHES IN EXPERIMENTAL T-2 TOXICOSES AT MICE AND USING FOOD ADDITIVE «VITAKORM»
Introduction. Among the many pollutants feed for livestock and poultry big danger are microscopic fungi producers of mycotoxins .Mycotoxins contamination feed received by the animals, can cause poisoning of animals, as well as their death. Existing physical, chemical and biological methods of processing contaminated feed are not effective and do not provide a solution to this problems.
Recently, much attention is paid to the special use of feed additives sorbents. This method is one of the most physiological, with no complications and it is easy to use.
The goal of the work. To investigate morphological changes in organs of mice with experimental T-2 toxicosis and use of feed additive "Vitakorm."
Materials and methods. In our research we have used food additive “Vitakorm” which is based on organic and inorganic components, as bentonite and fodder wheat bran, developed with laboratory staff mycotoxicology of Institute of veterinary medicine and Scientific and Production Complex “HYMTEHSERVYS”.
Laboratory research was held in the IVM NAAS and the Department of Pathological anatomy of NULES of Ukraine. For histological and histochemical studies were selected pieces from different parts of the heart, stomach, intestines, liver, kidneys and brain.
Results of the study and discussion. The histological experiments showed that is carrying out histological studies in mice that received T-2 toxin were set changes in the stomach. The structure of the mucosa was significantly impaired. In both layers of the muscle layer we observed granular dystrophy and swelling. In the duodenum and the ileum microscopical changes are the same as in the jejunum intestine, but less expressive. In the rectum microscopic changes were not found. Liver arteries and veins are dilated and full of blood. Most hepatocytes were in a state of granular dystrophy in the bile ducts were desquamation and destruction of epithelial cells. Histological researches of the kidneys show that their capsule is swollen. Tubular epithelium in all department sis in a state of granular dystrophy. The pancreas glandular epithelium of the state was in granular dystrophy. Histological studies of heart established that blood vessels are dilated, full of blood. Changes in endocardium and pericardium were not detected. In brain was found basophilia, hydropic degeneration and destruction of nerve cells. In spleen any microscopic changes has been established. In carrying out various histological studies of mice that received T-2 toxin with food, at a dose of 3.2 mg per kg of feed for 14 days and feed additive “Vitakorm” a dose of 2 g per kg of feed, we found that except liver and kidney, the microscopic structure of organs was similar as control animals. In the liver of mice that received T-2 toxin and additive particles in terms of liver hepatocytes revealed granular dystrophy. However, such changes as swelling of the parenchyma, destruction of the hepatocytes and liver tissue necrosis, as observed in mice that received only T-2 toxin, we have been found. In the study of kidney was found that the blood vessels of the stroma and glomerular capillaries expanded, full of blood. However, significant changes as observed in mice that received only T-2 toxin, have been found.
Conclusions and prospects further research. In histological researches we found microscopic changes in the liver andkidneys of mice treated with T-2 toxin and “Vitakorm” supplement significantlydifferent from those of mice that received only T-2 toxin. The research in vivoargue that the use of “Vitakorm” at a dose of 2 mg/kg is an effective approach tosolvethe mycotoxicoses problem.
Keywords: micromycetes, mycology researchers, patomorphologycaly researchers, food additive “Vitakorm”.