Veterynarna biotehnologija – Veterinary biotechnology, 2016, 28, 247-254 [in Ukrainian].

SACHUK R.N., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., ZHYHALUK S.V., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., ZBOZHYNSKA O.V., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., LUKYANYK I.N., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

Experimental Station of Epizootology of the Institute of Veterinary Medicine of NAAS

SUS H.V., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., MAGRELO N.V., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.,KATSARABA O.A., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

S.S.Gzhytsky Lviv National University of Veterinary Medicine and Biotechnology

PHYTOSPRAY EFFICIENCY FOR TREATMENT AND PREVENTION OF UDDER TEATS DERMATITISIN CATTLE

Introduction. Recently, for the treatment of various diseases in animals, including mastitis, were used herbal medicinal products that minimize negative effects on the immune status of animals and are environmentally safe compared to traditional drugs.

The goal of the work was to study the effect of therapeutic and preventive properties of aerosol drug “Fitosprey” of the udder teats and mastitis in cows.

Materials and methods. Preventive action and effectiveness of “Fitosprey” at dermatitis udder teats and mastitis in cows was studied in dairy farm “Mriya” in Rivne oblast on 37 lactating cows of black-motley breed of 2–8 years old by the results of bacteriological studies of udder skins swabs.

The biochemical properties of microorganisms defined in meat-pepton broth with 0.2% solution of glucose, alkaline-yolk agar, milk with 0.1% solution of methylene blue for the Hiss color row. Sensitivity of microflora tested regarding some natural essential oils were determined by agar disc method by G. Ibragimov. We studied microflora sensitivity to essential oils to enhance the antimicrobial activity (getting synergy effect).

Results of research and discussion.The most effective combination of bioactive compounds of plant origin and became part of the study drug “Fitosprey”. Study of antimicrobial activity of the drug showed that inhibition zone of Staphylococcus aureus was 34±0.3 mm, inhibition zone of the saprophytic Staphylococcus was 28±1.8 mm.

We investigated the preventive effect of “Fitosprey” under production environment. Inflammatory processes in cow’s research group observed in 8 (13.3%) teats and 1 cow (6.7%) had subclinical mastitis. We conducted macroscopic determination of regenerative and reparative properties of developed drug “Fitosprey”. Results have showed that on the 4th day after drug application inflammation on the skin of test animals decreased, as well as decreased tissue edema, damaged area was covered with dense young pink epithelial tissue. Swelling of tissues disappeared on 8–9th day, and healing occurred. The surface of the affected tissue palpation was painless and slightly tightened.

Conclusions and prospects for further research. Created drug “Fitosprey” based on the combination of essential oils of tea tree, cajeput, pine, juniper, clove and eucalyptus showed high efficiency in prevention diseases of adder teats skin and subclinical mastitis, regenerative and reparative action, showing no allergic and sensebilitive properties. After proper registration of the drug can enter the arsenal of veterinarian as an effective and environmentally safe means of preventive and therapeutic action in skin diseases in animals infectious origin.

Keywords: cow mastitis, prevention, essential oils, herbal spray, antimicrobial action, teats of the udder.

REFERENCES

  1. Ivashura, A.I. (1991). Sistema meroprijatij po bor'be s mastitami korov [The system of measures to eliminate mastitis of cows]. Moscow: Rosagropromizdat [in Russian].
  2. Popov, Y.G. (2004). Еtiopatogeneticheskaja rol' uslovno-patogennoj mikroflory pri massovyh boleznjah skota [Etiopatogenetic role of opportunistic pathogenic microflora in mass diseases of livestock]. Proceedings from The Modern problems of epizootology: mezhdunarodnaja nauchnaja konferencija (30 ijunja 2004 g) – International scientific conference. (pp. 204-207). Novosibirsk [in Russian].
  3. Danilov, M.S., & Vorobyov, A.L. (2004). Bentonitovyj fitogel' dlіa profilaktiki dermatitov soskov vymeni i mastita u korov. [Bentonite Phytogel for the prevention of dermatitis of the udder and mastitis in cows]. Vestnik Novosibirskogo gosudarstvennogo agrarnogo universiteta – Bulletin of the Novosibirsk State Agrarian University, 4 (20), 79-82 [in Russian].
  4. Kosenko, M.V., & Malik, O.H. (2001).  Veterynarni fitopreparaty: dovidnyk [Veterinary herbal: directory]. Lviv: Spolom. [in Ukrainian].
  5. Sachuk, R.M. (2015). Klinichne doslidzhennja diji ekologichno bezpechnogo preparatu “Fitosprej” pry shkirnyh zahvorjuvannjah domashnih m’jasoi'dnyh tvaryn [Clinical study of action of ecological safe preparation “Fitosprej” at skin diseases of domestic carnivores]. Veterynarni nauky: Nauk. visnyk L'vivs'kogo nac. u-tu vet. med. ta biotehnol. im. S.Z. G'zhyc'kogo –Scientific Bulletin of Lviv National University of Veterinary Medicine and Biotechnology named after SZ Gzhytsky,Vol. 17, 2, 1 (61), 297-301 [in Ukrainian].
  6. Sachuk, R.N. (2015). Netradicionnye metody lechenija kozhnyh zabolevanij domashnih plotojadnyh zhivotnyh [ Nontraditional methods of skin diseases treatment in domestic carnivores]. Proceedings from The Innovative development of young scientists – for the development of agro-industrial complex: IV mezhdunarodnaja konferencija (18-19 sentjabrja 2015 goda) – 4th International conference. Vol.1, 8, (pp. 499-502). Stavropol': Bjuro novostej [in Russian].
  7. Kostyshyn, E.E., Stefaniak, Yu.J., & Ivaniak, Y.I. (2003). Metodydiagnostykyilikuvanniatvaryn, hvoryhnamastyt : metodychnirekomendaciji[Methods of diagnosis and treatment of animals suffering from mastitis: guidance].Lviv: LNUVM after S.Z. Gzhytsky [in Ukrainian].
  8. Pro zatverdzhennya metodychnyh vkazіvok schodo viznachennya chutlivostі mіkroorganіzmіv do antibakterіalnih preparatіv [On Approval of guidelines for determining the sensitivity the of microorganisms to antibiotics]. Order of the Ministry of Health of Ukraine № 167 from 05.04.2007. K.: MOZ Ukrainy [in Ukrainian].
  9. Holt, J., Krieg, N., & Snit, P. (Eds.). (1997). Opredelytel' bakteryj Berdzhe [Determinant Burge]. (Vol. 1-2). M.: Mir [in Russian].
  10. Rokytskyy, P.F. (1973). Byolohycheskaya statistika[Biological Statistics]. Minsk: Vyshejshaja shkola [in Russian].

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