Veterynarna biotehnologija – Veterinary biotechnology, 2016, 28, 271-278 [in Ukrainian].
Ternopil Experimental Station of the Institute of Veterinary Medicine of NAAS
F.D. Ovcharenko Institute of Biocolloidal Chemistry
A POSTNATAL PATHOLOGY PREVENTION IN COWS BY SUPPOSITORIES CONTAINING COPPER NANOPARTICLES
Introduction. Improving the cows reproductive ability is extremely important for the livestock industry. Current conditions and care of animals require the development of new ways to influence the sexual function of cows and clear recommendations for their practical implementation.
It is known that in the first two or three days after calving reproductive system is most sensitive to infection by microorganisms. In this period, the myometrium contraction contributing to enhanced of lochia release, thus protecting the reproductive system from infection.
The goal of the work. Construct suppositories containing copper nanoparticles and examine their impact on processes of lipid peroxidation and antioxidant system.
Materials and methods of research.Experiment conducted in LLC “Agroprodservice-Invest” of Ternopil oblast in dairy cattle of Ukrainian black and white breed. Biochemical studies conducted in the laboratory of veterinary obstetrics and gynecology of Ternopil Research Station of Institute of Veterinary Medicine of NAAS.
Research of cows blood for content of lipid peroxidation products and antioxidant system performed at the beginning and the end of farmyard period, after suppositories containing copper nanoparticles application.
Results and discussion. Intensification of lipid peroxidation (LPO) is considered part of antioxidant stress that plays a significant role in the pathogenesis of many diseases. With LPO directly related to nonspecific adaptive reactions such as, the rate of cell division, work enzyme systems regulating membrane permeability and so on. In response to the development of inflammation in the body antioxidant system reduces the level of free radicals under physiological conditions. There is equilibrium between lipid peroxidation and antioxidant protection system.
After application of suppositories containing nanoparticles of copper in cows blood reduced the content of diene conjugates to 50.0% (p≤0.001) and the concentration of TBA active products 22.0% (p≤0.01).
Note the positive trend tounreliable increase catalase activity in blood after using suppositories containing copper nanoparticles. At the same time in cows of the control group was unlikely reducing lipid peroxidation products and increased activity of catalase.
It is known that free radicals are compounds lacking one electron in one of the molecules in the cells. If free radical formation occurs then the intracellular balance is disturbed, there is a chain reaction and new radicals penetrating weakened cell. In fact, it occurs in the uterus after discharge of afterbirth. The antioxidant properties have substances that can act as electron donors. Suppositories containing copper nanoparticles are donors of electrons, which determines their necessity while organism combating with free radicals on the organ’s level (uterus). Service time is an indicator of uterine involution and restore reproductive function of cows after calving. After application of suppositories containing copper nanoparticles in cows duration of the service period decreased in 1.9 times (p≤0.05) with 1.5 insemination index compared to the control group. Obtained data indicate a positive impact of suppositories containing copper nanoparticles for involution of the reproductive system.
Conclusions and prospects for further research. After applying suppositories containing nanoparticles of copper in calving day the content of diene conjugates decreases by 50.0% (p≤0.001), TBA active products by 22.0% (p≤0.01), which positively affect the course of postnatal period and facilitates service-shortened period to 62.5±2.1 days at insemination index 1.5.
The prospect of further research is to study the influence of prolonged use of suppositories containing copper nanoparticles for the development of gynecological diseases and fetal mortality.
Keywords: postnatal period, suppositories copper nanoparticles, lipid peroxidation, service-shortened period, insemination index.