Veterynarna biotehnologija – Veterinary biotechnology, 2018, 32(1), 50-55 [in Russian]. https://doi.org/10.31073/vet_biotech32(1)-04
BARYSAVETS D.S., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., ZUJKEVICH T.A., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., KOSTIUK N.I., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., STRELCHENIA I.I., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., TKALICH E.S., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
S.N. Vyshelesky Institute of Experimental Veterinary Medicine, Minsk, Republic of Belarus
KRASOCHKO P.A., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
The Vitebsk State Academy of Veterinary Medicine, Republic of Belarus
STUDY OF GROWTH NUTRIENT MEDIUM OF UNIDENTIFIEDCELL CULTURE AND SUBSTANTIATION OF THE COMPOSITION OF PREPARATIONS BASED ON THE PRODUCTS OF THEIR METABOLISM
Introduction. For the cultivation of animal cells, nutrient media having a complex composition are used. They are composed of high-quality, comparatively expensive raw materials with subsequent addition of nutritional and growth additives. This creates the prerequisites for using the spent nutrient medium containing the products of the metabolism of uninfected cell cultures, with the goal of creating veterinary drugs that have a common biotonic and immunomodulating effect, stimulating the growth and development of young animals.
Objective. To study the exchange of proteins, macro- and microelements of growth nutrient media before and after cultivation of monolayer cell cultures with high and low metabolic rate and theoretically substantiate the composition of preparations on the basis of growth media of uninfected cell cultures, select components and study their physicochemical properties.
Material and methods of research. When performing the research work, samples of growth nutrient media were used before and after cultivation of transplantable cell cultures. For the design of preparations based on growth culture media of transplantable cell cultures, the theoretical justification was carried out according to the following parameters: - provision of sterility; - the need to introduce sorbents into the formulation.
The results of the research and their discussion. When conducting studies on the study of protein metabolism, there was a decrease in the amount of total protein in growth nutrient media after culturing monolayer cell cultures in all test samples, depending on the terms of cultivation of the cell line. When conducting studies on the mass fraction of micro- and macroelements in growth nutrient media, it was established that in all samples except FGMS + Needle DMEM, the oscillations of micro- and macroelements are insignificant and range from 1 to 17%. For the design of laboratory samples of drugs selected components: - as preservatives – teotropin or formalin; - as sorbents – zeolite, cellulose, chitosan, hydroxyl.
Conclusions and prospects for further research. 1. Cultivation of monolayer cell cultures with high and low metabolic rate for 2–7 days leads to a decrease in the level of total protein in growth nutrient media by 7.04%–18.6%. 2. The content of micro- and macroelements in growth nutrient media during the cultivation of transplanted cell lines undergoes insignificant fluctuations within 1–17%, except for the medium of FGMS + Needle DMEM (cell culture 3KG). 3. The composition of the preparations is theoretically justified, for the design of laboratory samples.
Key words: nutrient media, cell cultures, veterinary preparations.
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