Bulletin "Veterinary biotechnology"

Veterynarna biotehnologija – Veterinary biotechnology, 2018, 32(1), 71-79 [in Ukrainian]. https://doi.org/10.31073/vet_biotech32(1)-07

VYGOVSKA L.M., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

National University of Life and Environmental Sciences of Ukraine, Ukrainian laboratory of quality and safety of agricultural products


Introduction. Etiotropic therapy for salmonellosis consists in the use of derivatives of penicillin and fluoroquinolones. In the Department of Microbiological Research, the Ukrainian Laboratory of Quality and Safety of Agricultural Products studies of the biological properties of epizootic strains and the study of their antibiotic susceptibility.

The goal of the work. Study of biological properties and sensitivity to antibiotics of epizootic strains of Salmonella spp. in S-, R-, L-forms.

Materials and methods. The biological properties of Salmonella spp were studied according to culture-morphological, enzymatic, antigenic properties, pathogenicity and antibiotic sensitivity to disodiphyseal method in accordance with EUCAST.

Results of research and discussion. According to the results of studies, there were isolated 59 strains of Salmonella spp. Strains in the R- and L-forms differed from the strains in the S-form with low levels of biological activity and loss of some properties. Salmonella spp. S-form showed resistance to most antibiotics. In R-, L-forms to penicillins, cephalosporins, aminoglycosides, and ftrochinolones there was an increase in sensitivity in S. cholerae suis, S.dublin strains; in S. typhimurium – a decrease in sensitivity. All cultures in the R-, L-forms significantly reduced the sensitivity to carbopenems. To macrolides and linzamides, changes in sensitivity were not recorded. The sensitivity of cultures to tetracyclines increased, with the exception of S. typhimurium in the R-form. Nitrofurans – marked a decrease in the sensitivity of S. ch.suis L-forms and increased in S. dublin L-forms. To azoles were marked reduction S. ch.suis in the R-form, other cultures-without changes.

Conclusions and prospects for further research. Strains of Salmonella spp. in S-form had typical culturally-morphological, enzymatic and pathogenic properties, antigenic structure; in the R- and L-forms differed from the strains in the S-form with low levels of biological activity and loss of some properties. Salmonella spp. exhibited resistance to most antibiotics from the studied groups; in the dissociation states there were some changes in the sensitivity of cultures to antibiotics. Further research will focus on determining the mechanisms of antibiotic resistance in the strains examined.

Keywords: salmonella, biological properties, antibiotic resistance, sensitivity.


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