Veterynarna biotehnologija – Veterinary biotechnology, 2018, 32(1), 92-98 [in Ukrainian]. https://doi.org/10.31073/vet_biotech32(1)-10

GUJVINSKA S.O., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., PALIY A.P., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

National Scientific Center “Institute of Experimental and Clinical Veterinary Medicine”

DETERMINATION OF ANTAGONISTIC AND ADHESIVE PROPERTIES OF LACTOBACTERIUM AND BIFIDOBACTERIUM

Aim. To study the biological properties of lactobacillus strains and bifidobacteria and to select promising microorganisms for the production of probiotic for poultry.

Materials and methods. The object of the research was 16 strains of lactobacterium and bifidobacterium which are stored in National Scientific Center «Institute of experimental and clinical veterinary medicine». Antagonistic activity of lactobacilli and bifidobacteria was determined by the method of delayed antagonism.

Results of research and discussion. Of the 16 strains examined, strains Lactbacillus plantarum 7–317 and Bifidobacterium adolescentis 17–316, which exhibited the highest antagonistic activity in relation to opportunistic microorganisms: Escherichia coli K 99, Str epidermidis, S. aureus, Salmonella dublin. Studies have shown that strains L. plantarum 37, L. casei 27, B. adolescentis 47, B. adolescentis 17–316, L. plantarum 7–317, L. plantarum 22, B. adolescentis 3, showed a high antagonistic activity, growth retardation zones fluctuated within 20.0 -25.0 mm; medium-active strains were B. adolescentis 23, St. lactis 15, B. longum 23 n, L. delbrueckii 42, L. plantarum 24 zones of growth retardation fluctuated within 10.0–19.0 mm; low-level strains were L. casei var. hamnosus 14, L. plantarum 24, L. casei var. rhamnosus 17, B. infantis 142. Thus, out of 16 strains, 7 (43.8%) showed high antagonistic activity, 5 strains (31.2%) showed an average antagonistic activity and 4 strains (25.0%) were low-level. It was found that using a mixture of probiotic cultures, the microflora of the intestine of the bird is corrected in the direction of increasing the beneficial microorganisms of the genus Lactobacillus from (2,5±0,07)х102CFU to (6,02±0,04) х106 cfu and the genus Bifidobacterium from (1,2±0,04) х102 CFU to (5,12±0,05) х102 CFU.

Conclusions and prospects for further research. As a result of the consistent selection of antagonistic properties, strains L. plantarum 7–317 and B. adolescentis 17–316 were selected, which are promising for the development on their basis of a domestic probiotic for poultry.

Keywords: lactobacterium, bifidobacterium, biological properties probiotics.

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