Veterynarna biotehnologija – Veterinary biotechnology, 2018, 32(1), 99-106 [in Ukrainian]. https://doi.org/10.31073/vet_biotech32(1)-11
ISHCHENKO L.M., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., ANDREEV I.V., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., PLOTNITSKA A.V., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., SHYNKARENKO L.M., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., KALAKAYLO L.I., е-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., KOLESNIKOVA T.P., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., USHKALOV V.O., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
National University of Life and Environmental Sciences of Ukraine, Ukrainian Laboratory of Quality and Safety of Agricultural Products
USE OF MOLECULAR GENETIC METHODS FOR QUALITY AND SAFETY CONTROL F PRODUCTION OF AGRO INDUSTRIAL COMPLEX
Introduction. In the Department of Molecular Biological Research, the Ukrainian Laboratory of Quality and Safety of Agricultural Products method of polymerase chain reaction is used to detection of genetically modified organisms in agricultural products and to DNA-identification of the species composition meat raw in products of the meat industry.
The goal of the work was to analyze and summarize the results of research detection of genetically modified organisms, identification of GM events, and DNA-identification of the species composition meat raw in sausages of the obtained in 2018.
Materials and methods. To detection of genetically modified organisms and identification of GM events were used samples of the commercial direction. To study DNA-identification of the species composition meat raw in sausages we used 12 different kinds of sausages which were purchased in the trading network.
Results of research and discussion. During 2018 were analyzed 356 samples of agricultural products for the detection of genetically modified organisms by the real-time PCR. We discovered 70 positive samples that representing 19,7 % of the total. It has been established that over the past three years, the number of positive samples in percentage terms increases yearly. The main GM events of plants that are present in Ukraine are the GM-soybean GTS 40–3-2 and the GM-rape GT 73. GM-event GTS 40–3-2 is also identified in feeds. It says that biotechnological soybean is used to produce feed. There were examined 12 samples different kind of sausages regarding the species composition of meat raw by real time PCR and found 5 samples(42,0 %), which did not correspond to the species composition of meat raw as declared by the producer.
Conclusions and prospects for further research. We have shown that in agricultural market in Ukraine are present genetically modified crops such as soybean, corn and rape (GM event soybeen GTS 40–3-2 and event MON 89788, rape GT 73). We have found that 5 samples from 12 (42,0 %) the species composition of meat raw did not correspond to the species composition of meat raw as declared by the producer. The obtained data testify to the violation of the technology of production of this kind of meat products.
Keywords: quality and safety of AIC products, molecular genetics methods of research, genetically modified plants, falsification of the species composition of meat products.
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