Bulletin "Veterinary biotechnology"

Veterynarna biotehnologija – Veterinary biotechnology, 2018, 32(1), 116-121 [in Ukrainian]. https://doi.org/10.31073/vet_biotech32(1)-13

KOVALENKO V.L.e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

State Scientific Control Institute of Biotechnology and strains (DNKIBSHM)

GARKAVENKO V.M.e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

State Scientific and Research Institute of Laboratory Diagnostics and Veterinary and Sanitary Expertise

KOVALENKO P.L.e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., PONOMAROVA S.A.e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

State Scientific-Research Control Institute of Veterinary Medicinal Products and Feed Additives, Lviv

THE STUDY OF ESSENTIAL OIL ANTIMICROBIAL ACTIVITY AND THEIR COMPOSITION ON MICROORGANISM

Introduction. Livestock farms suffer the greatest losses from diseases of the respiratory and gastrointestinal system. At the same time, there are many factors, both biotic and abiotic, which are the trigger and which significantly affect the further development of these diseases, efficiency of methods and means of their prevention and treatment. Therefore, the problems of prevention and elimination of animalsʹ bacterial diseases in industrial farms need to be solved in complex with the solution of other technological problems of the animal feeding and care.

The goal of the work. Selection and study of the efficiency of substances, components, necessary for the development of complex antibacterial agents. Study of their antimicrobial activity to microorganisms.

Materials and methods. Different antibiotic substances of benzalkonium chloride, silver nanoparticles and promising in this direction essential oils of plants: thyme, fir, eucalyptus were used for the work. Nutrient medias were used: NA, NB, Saburo medium, thioglycolic medium. For the determination of the antimicrobial activity of essential oils, the following test cultures were used: Microccoccus luteus ATCC9341, B. subtilis ATCC6633, B. cereus ATCC 10702, S. aureus 209-P, St. thermophiles, Clostridium perfringens, Klebsiella spp, Salmonella typhimurium, Pasterella multocida, Penicillium citrin, Penicillium urticae, Aspergillus flavus, Aspergillus ochraceus. In control tests the similarly infected test objects were sprayed with a physiological solution. In each experiment at least three test objects were used. Essential oils were applied to test objects at concentrations of 1:4000 – 1:8000. The efficiency of disinfection was determined by the presence or absence of test cultures, growth on nutrient medias during the incubation of crops in a thermostat at 37°C in 48 hours and 7 days.

Results of the research and discussion. The obtained data show high antimicrobial activity of essential oils of thyme, eucalyptus and fir, especially of their mixtures with benzalkonium chloride. The addition of silver nanoparticles significantly increases the antibiotic action of essential oil solutions. Using pure essential oils and mixtures of essential oils we observed the growth inhibition of S. aureus, St. thermophilus, P. multocida test microorganisms. Using the same mixtures with the addition of benzalkonium chloride and silver nanoparticles we observed the antimicrobial activity. We studied the minimum effective quantities and concentrations of essential oils to test microorganisms and some pathogenic isolates. Method of diffusion in agar was used. We applied 0.1 – 0.3 ml of essential oil to paper disks and measured the zones of growth inhibition of the studied cultures that were located within 13 – 36 mm. By the effectiveness of antibacterial action essential oils were determined in the following order: thyme, fir, eucalyptus. When we applied essential oils into nutrient media in the ratios of 1:10 – 1:200, a different degree of antibacterial activity was observed for the bacterial cultures. By the efficiency of fungicidal action the essential oils were determined in the following order: thyme, fir, eucalyptus.

Conclusions and prospects for further research. In experiments with S. aureus, St. thermophilus, P. multocida test-microorganisms the antibiotic action of essential oils of thyme, fir and eucalyptus was confirmed and the possibility of its enhancement by the addition of quaternary ammonium compounds was confirmed.

Keywords: Essential oils, microorganisms, benzalkonium chloride, nanoparticles, germicide.

REFERENCES

  1. Lorian, V. (1996). Antibiotics in laboratory medicine, 4th ed. Baltimore: Williams and Wilkins [in English].
  2. Weidemann, B. (1998). Evaluation of data from susceptibility testing. International journal of antimicrobial agents, 10, 218–219. [in English].
  3. Homzykov, O.M. (2006). Profilaktyka ta likuvannia hostrykh respiratornykh zakhvoriuvan teliat aerozoliamy aktyvnykh biolohichnykh preparativ [Prevention and treatment of acute respiratory diseases of calves with aerosols of active biological preparations]. Epizootology and prophylaxis of infectious diseases of cattle '06: Mizhnarodna nauk.-prakt. konf.: Tezy dopovidei – International scientific and practical conference: Abstracts of reports. (p. 25). Kyiv: NAU [in Ukrainian].
  4. Kovalenko, V.L., Yashchenko, M.F., & Chekhun, A.I. (2008). Vyznachennia efektyvnosti znezarazhennia dezinfektantom diamant na test-obiektakh [Determination of the effectiveness of decontamination of disinfectant diamond on test objects]. Mizhvidomchyi tematychnyi naukovyi zbirnyk UAAN. Kharkiv: Veterynarna medytsyna – Interagency thematic scientific collection UAAS. Kharkiv: Veterinary Medicine, 91, 220–222. [in Ukrainian].
  5. Hanaki, H., & Hiramatsu, K. (2003). Detection methods of glycopeptide-resistant Staphylococcus aureus. Susceptibility testing. Methods in Molecular Medicine vol 48: Antibiotic resistance methods and protocols. Humana press., 85–91 [in English].
  6. Kovalenko, V.L., & Nedosiekov, V.V. (2011). Kontseptsiia rozrobky ta vykorystannia kompleksnykh dezinfektantiv dlia veterynarnoi medytsyny: Monohrafiia [Concept of development and use of complex disinfectants for veterinary medicine: Monograph]. Kyiv [in Ukrainian].

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