Bulletin "Veterinary biotechnology"

Veterynarna biotehnologija – Veterinary biotechnology, 2018, 32(1), 156162 [in Ukrainian]. https://doi.org/10.31073/vet_biotech32(1)-20

NECHYPORENKO О.L., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., BEREZOVSKYY A.V.e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., PETROV R.V., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., FOTYN А.І., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

Sumy National Agrarian University

INVESTIGATION OF THE BIOCIDAL PROPERTIES OF THE DOMESTIC PREPARATION «DEZSAN»

Introduction. In order to prevent the occurrence of infectious diseases among poultry farms, a complex of measures is used, the key of which is disinfection of incubators, premises, equipment, inventory, working clothes and containers. Disinfection in poultry houses must be carried out taking into account the sustainability of the pathogens of the disease to the physico-chemical factors of the disinfectant and the potential danger to the environment. The urgent task facing veterinary medicine is the search for the latest disinfectants and detergents, based on the achievements of domestic and foreign practices, the use of the latest disinfectants that are harmless to humans and birds, environmentally safe and accessible to consumers.

The goal of the work. To study the biocidal properties of the new disinfectant "Dezsan", as well as the possibility of its use in the system of preventive measures during the conduct of veterinary and sanitary measures in livestock and poultry farms.

Materials and methods of research. Microflora sensitivity to disinfectant was determined by serial dilutions in a liquid nutrient medium. Determination of antimicrobial activity of disinfectants was carried out on pathogenic cultures that were isolated from various economic objects (air environment, corpses of birds and animals) of animal farms. As test objects, used tile, metal, plastic, brick and wood.

Results of research and discussion. It was found that a solution of "Dezsan" in 0.1% concentration in 100% of cases decontaminated test objects of iron and most types of microorganisms on the test facility from the wood, but did not cause 100% death of microbes on the plastered surface and bricks. In determining the antimicrobial action of the "Dezsan" solution in the next, higher concentration (0.25%), positive results of its influence on all test cultures placed on iron were obtained. In addition, this concentration of the solution showed a sufficiently high effective antimicrobial activity (over 99.3%) in relation to all test cultures of microorganisms that were applied to wood, plaster and bricks. In the next experiment, with a higher concentration of solution (0.5%) of the test agent, there was the majority of all microorganisms that were applied to all test objects (iron, wood, plastered surface and bricks). The obtained results indicate that a disinfectant at a concentration of 0.2 and 0.5% is an effective disinfectant. It is established that "Dezsan" has bactericidal and bacteriostatic action in relation to the means and can be used in the system of preventive measures during carrying out of veterinary and sanitary measures at farms.

Conclusions and prospects for further research: Full disinfectant effect on S. faecalis, C. fetus, C. jejuni, C. perfringens, P. aeruginosa, E. coli, P. vulgaris, S. enteritidis, A. fumigatus, Y. antterocolitica, S. pullorum-gallinarum, P. mirabilis is achieved at application of 0,25 – 0,5% solution "Dezsan". In the future, we plan to conduct a study of the drug "Dezsan", identified yogis irritating, skin-resorptive, cumulative and other properties.

Keywords: disinfectant, "Dezsan", strains, sensitivity of microorganisms

REFERENCES

  1. Pavlots'ka, L.F., Dudenko, N.V. & Dymytriyevych, L.R. (2007). Osnovy fiziolohiyi, hihiyeny ta problemy bezpeky kharchovykh produktiv: Navchal'nyy posibnyk [Fundamentals of Physiology, Hygiene and Food Safety Issues: A Manual]. Sumy: Universytet·s'ka knyha. [in Ukrainian].
  2. Sait «Tор 10 proyzvodyteley myasa ptyts v Ukrayne” [Site «Top 10 poultry producers in Ukraine»]. latifundist.com/rating/top-10-proizvoditelej-myasa-ptitsy-v-ukraine Retrieved from https://latifundist.com/rating/top-10-proizvoditelej-myasa-ptitsy-v-ukraine [in Russian].
  3. Sait «Derzhavnyy komitet statystyky Ukrayiny» [Site «State Committee of Statistics of Ukraine»]. ukrstat.gov.ua. Retrieved from http://ukrstat.gov.ua [in Ukrainian].
  4. Bessarabov, B.F., Sydorchuk, A.A. & Voronyn, E.S. (2007). Infektsyonnue bolezny zhyvotnukh [Infectious animal diseases ]. M.: Kolos [in Russian].
  5. Karysheva, A.F. (2002). Spetsial'na epizootolohiya: Pidruchnyk [Special epizootology: Tutorial ]. K.:Vyshcha osvita [in Ukrainian].
  6. Verbyts'kyy, P.I., Dostoyevs'kyy, P.P., Busol, V.O. et al (2004). Dovidnyk likarya veterynarnoyi medytsyny [Directory of Veterinary Medicine Doctor]. K.: Urozhay [in Ukrainian].
  7. Smith T.W. (2010). Sanitation: Cleaning and disinfectants. msucares.com/poultry/ diseases/sanitation.html. Retrieved from http://msucares.com/poultry/diseases/sanitation.html.
  8. Rutala, W.A. & Weber, D.J. (2013). Disinfectants used for environmental disinfection and new room decontamination technology. Am J Infect Control., 41, 36–41.
  9. Maris, P. (1995). Modes of action of disinfectants. Rev Sci Tech.,14, 47–55.
  10. Hoult, Dzh. (1997). Kratkiy operdelitel bakteriy Berdzhi [Short Berdzhi bacteria assayer]. M.: Mir [in Russian].
  11. Kotsyumbas, I.YA., Malyk, O.H., Patereha, I.P. et al. (2006). Doklinichni doslidzhennya veterynarnykh likarskykh zasobiv [Pre-clinical studies of veterinary medicinal products]. Lviv: Triada plyus [in Ukrainian].

Download full text in PDF