Bulletin "Veterinary biotechnology"

Veterynarna biotehnologija – Veterinary biotechnology, 2018, 32(1), 174-181 [in Ukrainian]. https://doi.org/10.31073/vet_biotech32(1)-23

OROBCHENKO O., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., ROMAN’KO M.e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., KUTSAN O.e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

National scientific center "Institute of Experimental and Clinical Veterinary Medicine"


Introduction. Physiologically grounded feeding of animals and poultry provides a receipt of genetically engineered productivity, as well as economic efficiency of the industry. Herewith the constant problem is the balancing of mineral and biologically active substances. Colloidal dispersions of nanoparticles of metals, which have high chemical stability and biocompatibility are of considerable interest for experimental studies, therefore scientific support in this direction is definitely necessary. In previous studies, biotic and toxic doses of nanocomposites of metals (NcMe: Ag, Cu, Fe, MnО2) were determined on the white rats, which became a prerequisite for conducting toxicological experiments on productive poultry – laying hens. Thus, the mechanism of toxic effect of NcMe in a dose of 4.0 mg/kg and adaptogenic action has been proved – at a dose of 0.3 mg/kg of the body weight of the chicken organism and the incubation quality of eggs, which was manifested by increased fertility, the breeding of conditioned growing birds, the weight of the bred chickens in comparison with metal salts.

The goal of the work was to study the clinical parameters of the blood of day-old chickens in the conditions of transovarial action of NcMe in comparison with the salts of corresponding metals.

Materials and methods. A study on the effect of NcMe on the development of chicken embryos was carried out on cocks (n=4) and laying hens (n=24) cross ''Highsex White'', 365 days old, weighing 1.2–1.6 kg, from which 4 groups with sex ratio of cocks to hens (1:6) were formed by the analogy principle. Birds of the control group additionally received distilled water into the feed, birds of the experimental groups during 37 days daily received additives to the feed: I group – a solution of a mixture of metal salts in a dose of 0.3 mg/kg, II group – NcMe in a dose of 0.3 mg/kg and III group – NcMe in a dose of 4.0 mg/kg of body weight. Starting from the 30th day, during the week, the eggs were collected from birds of each group and placed for incubation, after hatching of chickens they were examined clinically and then blood samples were taken for hematological studies during chloroform anesthesia and decapitation. The statistical processing of the research results was carried out using the Microsoft Excel 2003 application package (for Windows XP).

Results of research and discussion. It has been proved that the introduction of metal salts leads to a decrease in the total hemoglobin content on average by 9.8% (P<0.05) against the background of the physiological values of the number of red blood cells and leukocytes in the blood of chickens. Administration of NcMe at a dose of 0.3 mg/kg of body weight leads to an increase in the number of erythrocytes and total hemoglobin content on average by 35.6 and 10.2% (P<0.05), and at a dose of 4.0 mg/kg of body weight – only general hemoglobin by 9.9% (P<0.05). Introduction to the diet of laying hens NcMe supplements in both doses does not affect the level of leukocytes in the blood of hatched day-old chickens.

Conclusions and prospects for further research. It should be noted that the hemotoxic effect of the experimental sample NcMe in the conditions of transovarial action concerns only the cells of "red" blood, according to the mechanism of development it is unidirectional with such for the corresponding metals in the form of salts and is dose-dependent.

Keywords: day-old chickens, nanocomposite of metals, metal salts, clinical indicators of blood.


  1. Miroshnichenko, I.V., Boyko, I.A. & Kornienko, S.A. (2008). Effektyvnost' prymenenyya marhantsa tsytrata v kombykormakh tsyplyat-broylerov [The effectiveness of the use of manganese citrate in mixed fodders of broiler chickens]. Dostizheniye nauki i tekhniki APK – Advances in science and technology agriculture, 6, 45–47. [in Russian].
  2. Satyukova, L.P. (2015). Vlyyanye makro- y mykronutryentov na protsess obmena veshchestv v orhanyzme ptytsy [The influence of macro- and micronutrients on the metabolic process in the body of birds]. Problems of veterinary sanitation, hygiene and ecology – Problems on Veterinary Sanitation, Hygiene and Ecology (Journal), 3(15), 94–97. [in Russian].
  3. West, J.L. & Halas, N.J. (2000). Application of nanotechnology to biotechnology Current Opinion in Biotechnology. Current Opinion in Biotechnology, 11, 215–217.
  4. Bawa, R. (2008). Nanoparticle-based therapeutics in humans: a survey. Nanotechnology Law & Business, 5(2), 135–155.
  5. Sahoo, S.K., Parveen, S. & Panda, J.J. (2007). The present and future of nanotechnology in human health care. Nanomedicine, 3, 20–31.
  6. Chekman, І.S. (2011.) Nanopharmacologiya. [Nanopharmacology.] K.: Zadruga [in Ukrainian].
  7. Eleseev, A.A. & Lukashin, A.V. (2010). Funktsyonal'nue nanomateryalu. [Functional Nanomaterials.] M.: FIZMATLIT [in Russian].
  8. Orobchenko, A.L., Roman’ko, M.Ye. & Kutsan, A.T. (2014). Eksperymental'no-teoretycheskoe obosnovanye prymenenyya nanokompozyta metallov (Ag, Cu, Fe y dvuokys' Mn) dlya kur-nesushek pry uslovyy khronycheskoho postuplenyya s kormom (obobshchenye eksperymental'nukh yssledovanyy). [Experimental and theoretical basis for the use of nanocomposite (Ag, Cu, Fe and Mn dioxide) for laying hens under the conditions of chronic receipt with feed (a generalization of experimental studies)]. Veterinaria, Zootekhnia i Biotekhnologiya − Veterinary, zootechny and biotechnology, 12, 32–40. [in Russian].
  9. Nanokompozyt metaliv, yak potentsiynyy komponent biopreparativ i kormovykh dobavok dlya tvaryn [Metal nanocomposites as a potential component of biological products and feed additives for animals] Pat. 92804 IPC (2014.01) B01J 13/00, C01G 49/00, C10L 10/00 [in Ukrainian].
  10. Orobchenko, A.L., Roman’ko, M.Ye., Kutsan, A.T. & Breslavets, V.A. (2015). Embriotoksychnist' nanokompozytu (Ag, Cu, Fe i dvookys Mn) i soley metaliv za umov khronichnoho yikh nadkhodzhennya z kormom v orhanizm kurey-nesuchok. [Embryotoxicity nanocomposite (Ag, Cu, Fe and dioxide Mn) and metal salts chronic conditions they become available in the diet of laying-hens organism.] «Veterinarna medicina» mіzhvіdomchij tematichnij naukovij zbіrnik − «Veterinary medicine» inter-departmental subject scientific collection, 100, 187–190. [in Ukrainian].
  11. European Convention for the Protection of Vertebrate Animals used for Experimental and Other Scientific Purposes (Strasbourg, March 18, 1986). Retrieved from: http://zakon2.rada.gov.ua/laws/show/994_137 [in Ukrainian].
  12. Vlizlo, V.V. (2012). Laboratorni metody doslidzhen' u biolohiyi, tvarynnytstvi ta veterynarniy medytsyni : dovidnyk. [Laboratory methods of research in biology, veterinary medicine: a guide.] Lviv.: SPOLOM [in Ukrainian].
  13. Shamsutdinova, I.R. & Derkho, M.A. (2015). Yzmenenyya pokazateley krovi laboratornukh zhyvotnukh pry vvedenyy nanochastyts serebra. [Changes in the blood of laboratory animals when administered silver nanoparticles.]. Izvestija Orenburgskogo gosudarstvennogo universiteta (Veterinarija).− News of the Orenburg state agrarian university (veterinary science), 6(56), 122–124. [in Russian].
  14. Shamsutdinova, I.R. & Derkho, M.A. (2015). Yzmenenyya morfolohycheskykh pokazateley laboratornukh zhyvotnukh pry vvedenyy nanochastyts serebra per os. [Changes of blood morphological parameters of laboratory animals when introducing silver nanoparticles per os.] AIC Russia, 73, 166–170. [in Russian].
  15. Vishnyakov, A.I., Ushakov, A.S. & Lebedev, S.V. (2011). Osobennosty kostnomoz·hovoho krovetvorenyya pry vvedenye nanochastyts medy per os y intramuscularly. [Features of bone marrow hematopoiesis with the introduction of copper nanoparticles per os and intramuscularly.] Vestnik mjasnogo skotovodstva − The Herald of Beef Cattle Breeding, 2(64), 96−102. [in Russian].
  16. Yausheva, E.V. & Miroshnikov, S.A. (2015). Produktyvnoe deystvye sovmestnoho yspol'zovanyya preparatov nanochastyts zheleza y arhynyna v pytanyy tsuplyat-broylerov. [Productive action sharing drugs iron nanoparticles and arginine in the diet of broiler chickens] Izvestija Orenburgskogo gosudarstvennogo agrarnogo universiteta − News of the Orenburg state agrarian university, 5(55), 158–160. [in Russian].
  17. Busol, V.A. & Sytnik, M.G. (2013). Vplyv spozhyvannya nanokarboksylativ hermaniyu i zaliza na hematolohichni ta biokhimichni pokaznyky krovi kurchat-broyleriv. [Effect of germanium nanokarboksilats consumption and iron on hematological and biochemical blood parameters of broiler chickens.] Naukovi praci Pivdennogo filialu Nacional'nogo universytetu bioresursiv i pryrodokorystuvannja Ukrai'ny «Kryms'kyj agrotehnologichnyj universytet». Serija «Veterynarni nauky» − Crimean Agriculture technology University» Scientific works of the Southern Branch of the National University of Life and Environmental Sciences of Ukraine. Series «Veterinary science», 151, 160–165. [in Ukrainian].

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