Bulletin "Veterinary biotechnology"

Veterynarna biotehnologija – Veterinary biotechnology, 2018, 32(1), 174-181 [in Ukrainian]. https://doi.org/10.31073/vet_biotech32(1)-23

OROBCHENKO O., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., ROMAN’KO M.e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., KUTSAN O.e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

National scientific center "Institute of Experimental and Clinical Veterinary Medicine"

CLINICAL INDICES OF ONE DAY-OLD CHICKENS' BLOOD UNDER THE CONDITIONS OF TRANSOVARIAL ACTION OF METAL NANOCOMPOSITE (AG, CU, FE, MNО2) IN COMPARISON WITH METAL SALTS

Introduction. Physiologically grounded feeding of animals and poultry provides a receipt of genetically engineered productivity, as well as economic efficiency of the industry. Herewith the constant problem is the balancing of mineral and biologically active substances. Colloidal dispersions of nanoparticles of metals, which have high chemical stability and biocompatibility are of considerable interest for experimental studies, therefore scientific support in this direction is definitely necessary. In previous studies, biotic and toxic doses of nanocomposites of metals (NcMe: Ag, Cu, Fe, MnО2) were determined on the white rats, which became a prerequisite for conducting toxicological experiments on productive poultry – laying hens. Thus, the mechanism of toxic effect of NcMe in a dose of 4.0 mg/kg and adaptogenic action has been proved – at a dose of 0.3 mg/kg of the body weight of the chicken organism and the incubation quality of eggs, which was manifested by increased fertility, the breeding of conditioned growing birds, the weight of the bred chickens in comparison with metal salts.

The goal of the work was to study the clinical parameters of the blood of day-old chickens in the conditions of transovarial action of NcMe in comparison with the salts of corresponding metals.

Materials and methods. A study on the effect of NcMe on the development of chicken embryos was carried out on cocks (n=4) and laying hens (n=24) cross ''Highsex White'', 365 days old, weighing 1.2–1.6 kg, from which 4 groups with sex ratio of cocks to hens (1:6) were formed by the analogy principle. Birds of the control group additionally received distilled water into the feed, birds of the experimental groups during 37 days daily received additives to the feed: I group – a solution of a mixture of metal salts in a dose of 0.3 mg/kg, II group – NcMe in a dose of 0.3 mg/kg and III group – NcMe in a dose of 4.0 mg/kg of body weight. Starting from the 30th day, during the week, the eggs were collected from birds of each group and placed for incubation, after hatching of chickens they were examined clinically and then blood samples were taken for hematological studies during chloroform anesthesia and decapitation. The statistical processing of the research results was carried out using the Microsoft Excel 2003 application package (for Windows XP).

Results of research and discussion. It has been proved that the introduction of metal salts leads to a decrease in the total hemoglobin content on average by 9.8% (P<0.05) against the background of the physiological values of the number of red blood cells and leukocytes in the blood of chickens. Administration of NcMe at a dose of 0.3 mg/kg of body weight leads to an increase in the number of erythrocytes and total hemoglobin content on average by 35.6 and 10.2% (P<0.05), and at a dose of 4.0 mg/kg of body weight – only general hemoglobin by 9.9% (P<0.05). Introduction to the diet of laying hens NcMe supplements in both doses does not affect the level of leukocytes in the blood of hatched day-old chickens.

Conclusions and prospects for further research. It should be noted that the hemotoxic effect of the experimental sample NcMe in the conditions of transovarial action concerns only the cells of "red" blood, according to the mechanism of development it is unidirectional with such for the corresponding metals in the form of salts and is dose-dependent.

Keywords: day-old chickens, nanocomposite of metals, metal salts, clinical indicators of blood.

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