Bulletin "Veterinary biotechnology"

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Veterynarna biotehnologija – Veterinary biotechnology, 2018, 32(1), 182-190 [in Ukrainian]. https://doi.org/10.31073/vet_biotech32(1)-24

PINCHUK N.G., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., CHUMACHENKO V.V.

State Scientific Control Institute of Biotechnology and strains (DNKIBSHM)

STUDY OF THE SUSCEPTIBILITY OF ERYSIPELOTHRIX RHUSIOPATHIAE ISOLATED TO ANTIBACTERIAL DRUGS BY THE AGAR DILUTION METHOD

Introduction. The main purpose of determining the sensitivity of microorganisms to antibacterial drugs (ADP) is to predict their efficiency in the treatment of infectious diseases of animals. Currently, data on the sensitivity of epizootic isolated Er. rhusiopathiae are not available in Ukraine to ABP and the presence / absence of antibiotic resistance in these bacteria.

The goal of the work was the development of basic principles of agar successive dilution method and the study of the sensitivity of epizootic isolated Er. rhusiopathiae to ABP by agar successive dilution method.

Materials and methods. Materials for the study were 47 isolated of the pathogens of pigs, isolated from the pathological material of dead pigs from different regions of Ukraine. Studying the sensitivity of isolated Er. rhusiopathiae was administered to 17 ABP: PC-G, APC, KM, GEN, NEO, EM, DSM, OTC, DOX, CZ, SZ, SDM, ENR, NX, LE, LCM, CP. The following experimental nutrient media: MHA, TCA, HBA and Hottinger meat-pepton agar (180–200 mg% of amine nitrogen) were used in various experiments using agar successive dilution method in variants: without additives; with the addition of 5% of the DHB; 0.1% Tween 80.

Results of research and discussion. It has been established that the TSA with a 0.1% Tween-80 increase in growth retardation zones is more distinct and well expressed compared to those obtained on the TSA with the addition of 5% of the DHB. In the future, in studying the sensitivity of epizootic isolated Er. rhusiopathiae to ABP by agar successive dilution method, we used TSA with 0.1% Tween-80 at a temperature of (35 ± 0.2) °C for 24 hours. The results of the studies indicate that all epizootic isolated Er. rhusiopathiae (47) were highly sensitive to PC-G, APC, EM and 100% resistant to KM, CZ, SZ, SDM. The number of isolated resistant to GEN, NEO, DSM, OTC, DOX, LCM and CP was 46 (97.9%), 43 (91.5%), 31 (66.0%), 27 (57.4%), 18 (38.1%), 36 (76.6%) and 42 (89.4%) respectively. In some of the isolated from different regions of Ukraine, we found resistance to ENR, NX and LE: 3 (6.4%), 34 (72.3%) and 32 (68.1%), respectively.

Conclusions and prospects for further research. Sensitivity of 47 epizootic isolated Er. rhusiopathiae was studied to 17 ABP by agar successive dilution method. Fluoroquinolone-resistant epizootic isolated Er. rhusiopathiae detected to ENR, NX and LE.

Keywords: antibacterial drugs (ABP), antibiotic resistance, antibiotic susceptibility, swine erysipelas, minimal inhibitory concentrations (MIC).

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