Bulletin "Veterinary biotechnology"

Veterynarna biotehnologija – Veterinary biotechnology, 2018, 32(1), 202-206 [in Ukrainian]. https://doi.org/10.31073/vet_biotech32(1)-26

PUSTOVIT N.A., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., PINCHUK N.G., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., KUDRYAVCHENKO A.P., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

State Scientific Control Institute of Biotechnology and strains (DNKIBSHM)


Introduction. Campylobacteriosis is a group of zoonotic bacterial infections caused by bacteria belonging to the genus Campylobacter. In humans, they occur mainly in the acute form of the disease and are characterized by lesions of the gastrointestinal tract, with a tendency to generalize the process with the development of septicemia and the defeat of various organs and systems. Pathogens of Campylobacteriosis are widespread in nature. The incidence of Campylobacteriosis is recorded in the form of sporadic cases, epidemic foci with food and water through the transmission of infection. In some countries, Campylobacteriosis is a leading etiological agent of acute intestinal infections, ahead of Salmonella and Shigellosis.

The goal of the work was to investigate the ultrastructure of isolates of Campylobacter isolated from poultry products.

Materials and methods of research. The isolated isolates were cultured on a dense blood medium (Columbia agar with 10% defibrillated sheep's blood). Cultivated at 37 °C and 7% CO2 for 48–72 h. The isolated isolates were removed from culture media and emulsified in a phosphate buffer saline solution. Then the material was prepared according to the methods for electron microscopic examination.

Results of research and discussion. The choice of the method of preparation of material for the purpose of manufacturing from it preparations for electron microscopic examination depends on the concentration of the microorganism in the suspension, the degree of its contamination by foreign ballast substances and morphology of the pathogen. The basis for using direct electron microscopy of the materials studied was taken.

Conclusions and prospects for further research. An electron microscopy carried out a morphological study of the ultrastructure of the isolates of Campylobacter isolated from poultry products. The mechanism of coccal cell formation remains unknown, but our observation suggests a progressive change in the microorganism associated with degeneration of the cell wall and a change in the conditions of cultivation, which allows further research in this direction.

Keywords: campylobacter, pathogen, distribution, intestinal infection, electron microscopy.


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