Bulletin "Veterinary biotechnology"

Veterynarna biotehnologija – Veterinary biotechnology, 2018, 32(1), 225-232 [in Ukrainian]. https://doi.org/10.31073/vet_biotech32(1)-29

RUBLENKO I.e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

Bila Tserkva National Agrarian University

SKRYPNYK V.

State Scientific and Research Institute of Laboratory Diagnostics and Veterinary and Sanitary Expertise

PINCHUK N., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., PUSTOVIT N., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.RUBLENKO N.

State Scientific Control Institute of Biotechnology and strains (DNKIBSHM)

DETERMINATION OF THE STABILITY OF THE BIOLOGICAL PROPERTIES OF THE VACCINE STRAIN BAC. ANTHRACIS UA-07 IN PRODUCTION CONDITIONS

Introduction. In the world, among animals and humans, there is the problem of infectious anthrax disease. The use of modern vaccines made from harmless, avirulent strains – is considered as a direction of development of prevention, which will improve the epizootic situation.

The goal of the work was to study the stability of the biological properties of the Bacillus anthracis UA–07 vaccine strain under production conditions.

Materials and methods. Vaccine strain Bacillus anthracis UA–07, microorganism of the genus Bacillus, species anthracis, immobile, rod-positive gram, optional anaerobes. On a dense nutrient medium, Hottinger grew in the form of R-shaped colonies.

Results of research and discussion. As a result of the "pearl necklace" test, spherical forms of the cells of the pathogen Bacillus anthracis UA–07, located in the form of chains resembling a pearl necklace, were found on the medium containing penicillin. On the control medium without penicillin cells Bac. anthracis formed long chains of typical sticks. Twenty-fold passages of the strain studied through the nutrient medium of the MPA with serum did not lead to the formation of a capsule by the pathogen Bacillus anthracis UA–07. Ten-fold passages of the Bacillus anthracis UA–07 vaccine strain caused by the bacteria in a dose of 10 billion/cm3 did not result in the appearance of a capsule in the bacteria found on the studied smears and sputum preparations, liver, lung, and heart blood. Investigations on guinea pigs, with the introduction of 10 billion cultures, found that Bacillus anthracis UA–07 after a 3-time repetition of the previous passage was not isolated from the body of mollusks.

Conclusion and prospects for further research. Vaccine strain Bacillus anthracis UA–07 has stable biological properties and can be used in further studies to create the vaccine.

Keywords: anthrax, stability, Bacillus anthracis, mice, guinea pigs.

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