Bulletin "Veterinary biotechnology"

Veterynarna biotehnologija – Veterinary biotechnology, 2018, 32(1), 225-232 [in Ukrainian]. https://doi.org/10.31073/vet_biotech32(1)-29

RUBLENKO I.e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

Bila Tserkva National Agrarian University


State Scientific and Research Institute of Laboratory Diagnostics and Veterinary and Sanitary Expertise

PINCHUK N., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., PUSTOVIT N., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.RUBLENKO N.

State Scientific Control Institute of Biotechnology and strains (DNKIBSHM)


Introduction. In the world, among animals and humans, there is the problem of infectious anthrax disease. The use of modern vaccines made from harmless, avirulent strains – is considered as a direction of development of prevention, which will improve the epizootic situation.

The goal of the work was to study the stability of the biological properties of the Bacillus anthracis UA–07 vaccine strain under production conditions.

Materials and methods. Vaccine strain Bacillus anthracis UA–07, microorganism of the genus Bacillus, species anthracis, immobile, rod-positive gram, optional anaerobes. On a dense nutrient medium, Hottinger grew in the form of R-shaped colonies.

Results of research and discussion. As a result of the "pearl necklace" test, spherical forms of the cells of the pathogen Bacillus anthracis UA–07, located in the form of chains resembling a pearl necklace, were found on the medium containing penicillin. On the control medium without penicillin cells Bac. anthracis formed long chains of typical sticks. Twenty-fold passages of the strain studied through the nutrient medium of the MPA with serum did not lead to the formation of a capsule by the pathogen Bacillus anthracis UA–07. Ten-fold passages of the Bacillus anthracis UA–07 vaccine strain caused by the bacteria in a dose of 10 billion/cm3 did not result in the appearance of a capsule in the bacteria found on the studied smears and sputum preparations, liver, lung, and heart blood. Investigations on guinea pigs, with the introduction of 10 billion cultures, found that Bacillus anthracis UA–07 after a 3-time repetition of the previous passage was not isolated from the body of mollusks.

Conclusion and prospects for further research. Vaccine strain Bacillus anthracis UA–07 has stable biological properties and can be used in further studies to create the vaccine.

Keywords: anthrax, stability, Bacillus anthracis, mice, guinea pigs.


  1. Povіdomlennja pro zahvorjuvannja. Bjuleten' pro іnfekcіjnі zahvorjuvannja 01–31.2017r. [Reporting the disease. Newsletter on infectious diseases 01–31.2017] vetlabresearch.gov.ua Retrieved from: http://vetlabresearch.gov.ua/news/?ELEMENT_ID=1922 [in Ukrainian].
  2. Anthrax, Mozambique. Information received on 03.10.2017 from Dr Américo Da Conceicao, National Director, Veterinary Services, Ministry of Agriculture, Maputo, Mozambique www.oie.int Retrieved from: http://www.oie.int/wahis_2/public/wahid.php/Reviewreport/Review?reportid=24858.
  3. Anthrax. Weekly disease information. www.oie.int Retrieved from: http://www.oie.int/wahis_2/public/wahid.php/Diseaseinformation/WI/index/newlang/en.
  4. Mwakapeje, E.R., Høgset, S., Fyumagwa, R., Nonga, H.E., Mdegela, R.H., Skjerve E. et al. (2018). Anthrax outbreaks in the humans – livestock and wildlife interface areas of Northern Tanzania: a retrospective record review 2006–2016. BMC Public Health, 18, 106.
  5. Liang, X.D. (2015). Anthrax surveillance situation in China. Disease Surveillance., 14(2), 69–71.
  6. Chen, W-J., Lai, Sh-J., et al. (2016). Mapping the distribution of Anthrax in Mainland China, 2005–2013 PLoS Neglected Tropical Diseases. 10(4), 1–15.
  7. Fasanella, A., Di, T.P., Garofolo, G., Colao, V., Marino, L., Buonavoglia, D., et al. (2013). Ground anthrax bacillus refined isolation (GABRI) method for analyzing environmental samples with low levels of bacillus anthracis contamination. BMC Microbiol., 13, 113–167.
  8. European Centre for Disease Prevention and Control (ECDC). ecdc. europa.eu. Retrieved from: http://www.ecdc. europa.eu/en/Pages/home.aspx.
  9. Blackburn, J.K., Odugbo, M.O., Ert, M.V., O’Shea, B., Mullins, J., et al. (2015). Bacillus anthracis diversity and geographic potential across Nigeria, Cameroon and Chad: further support of a novel west african lineage. Рlos. Neglected Tropical Diseases, 9(9) Retrieved from: http://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0003931.
  10. Munyua, P., Bitek, A., Osoro, E., Pieracci, E.G., Muema, J., et al. (2016). Prioritization of Zoonotic Diseases in Kenya, Рlos. Neglected Tropical Diseases, 10(5). Retrieved from: http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0161576 [in English].
  11. Kracalik, I., Malania, L., Imnadze, P. & Blackburn, J.K. (2015). Human anthrax transmission at the urban–rural interface, Georgia. The American Journal of Tropical Medicine and Hygiene, 93(6), 1156–1159.
  12. Kracalik, I.T., Kenu, E., Ayamdooh, E.N., Cudjoe, E.A. et al. (2017). Modeling the environmental suitability of anthrax in Ghana and estimating populations at risk: Implications for vaccination and control. Рlos. Neglected Tropical Diseases, 10(5). Retrieved from: http://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0005885.
  13. Afshar, P., Hedayati, M.T., Aslani, N. & Khodavaisy, S. (2015). First autochthonous coinfected anthrax in an immunocompetent patient. Case Reports in Medicine, 2015. Retrieved from: https://www.hindawi.com/journals/crim/2015/325093/abs/.
  14. Berger, T., Kassirer, M. & Aran, A.A. (2014). Injectional anthrax–new presentation of an old disease. Eurosurveillance, 19(32). Retrieved from: https://www.eurosurveillance.org/content/10.2807/1560–7917.ES2014.19.32.20877.
  15. Hendricks, K.A., Wright, M.E., Shadomy, S.V., Bradley, J.S., Morrow, M.G., Pavia, A.T. et al. (2014). Centers for disease control and prevention expert panel meetings on prevention and treatment of anthrax in adults. Emerg Infect Dis, 20(2) Retrieved from: http://dx.doi. org/10.3201/eid2002.130687.
  16. Barnett, D.J., Balicer, R.D., Blodgett, D.W., Everly, G.S., Omer, S.B. et al. (2005). Applying risk perception theory to public health workforce preparedness training. Public Health Manag Pract, 33–37, 833–837.
  17. OIE-Listed diseases, infections and infestations in force in 2015. www.oie.int. Retrieved from: http://www.oie.int/en/animal-health-in-the-world/oie-listed-diseases-2015/.
  18. Bezymennyi, M., Bagamian, K.H., Barro, A., Skrypnyk, A., Skrypnyk, V., Blackburn, J.K. et al (2014). Spatio-temporal patterns of livestock anthrax in Ukraine during the past century (1913–2012) Applied Geography, 54, 129–138.
  19. Rublenko, І.O. & Skripnik, V.G. (2016). Analіz danih epіzootichnih spalahіv sibіrki na teritorіi Ukraini (perіod 1994 – 2016 rr.) [Analysis of the data of epizootic outbreaks of anthrax on the territory of Ukraine (1994 – 2016)]. Nauk. vіsnik vet. med. Zbіrnik naukovih prac' – Scientific Herald of Veterinary Medicine. Collection of scientific works, 1(127), 87–95 [in Ukrainian].
  20. Skripnik, V.G., Rublenko, І.O., Garkavenko, T.O. et al. (2015). Laboratorna dіagnostika sibіrki tvarin, іndikacіja zbudnika z patologіchnogo ta bіologіchnogo materіalu, sirovini tvarinnogo pohodzhennja ta ob’єktіv navkolishn'ogo seredovishha [Laboratory diagnosis of anthrax of animals, indication of a pathogenic agent from pathological and biological material, raw materials of animal origin and objects of the environment]. Kyiv: DVFSSU [in Ukrainian].

Download full text in PDF