Bulletin "Veterinary biotechnology"

Veterynarna biotehnologija – Veterinary biotechnology, 2018, 32(1), 238-244 [in Ukrainian]. https://doi.org/10.31073/vet_biotech32(1)-31

SAVINOVA I.V., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., KLESTOVA Z.S.e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

State Scientific Control Institute of Biotechnology and strains (DNKIBSHM)

NEW BIOTECHNOLOGICAL APPROACHES FOR THE INVESTIGATION OF WARM-BLOODED ANIMALS’ VIRUSES USINGOF ECTOTHERMIC ANIMALS CELLS

Introduction. Domestic and wild animal population movements are important in the spread of disease. Understanding the volume of these movements and the risks associated with them is fundamental in elucidating the epidemiology of these diseases, some of which might entail zoonotic risks. The importance of the worldwide animal trade is reviewed and the role of the unregulated trade in animals is highlighted. A range of key examples are discussed in which animal movements have resulted in the introduction of pathogens to previously disease-free areas. Measures based on heightened surveillance are proposed that mitigate the risks of new pathogen introductions. Cell culture is essential for virus replication and isolation and this is a useful diagnostic tool to investigate the viral ecology, biology and evolution.

The goal of the work. The aim of this study was to investigate a new stable cell line from the pool of internal parenchymal organs of the newborn turtle (Trachemys scripta elegance) for viral research.

Materials and methods of research. There are BHK-21(baby hamster kidney), MDBK (Bos taurus kidney) and TF (internal parenchymal organs of the newborn turtle Red-eared slider) cell lines. There are DNA and RNA-containing viral models of the families Herpesviridae (Aujeszky's disease virus) and Rhabdoviridae (Vesicular stomatitis virus, (Indiana)).

Results of research and discussion. The sensitivity of the newly obtained cell line TF from the cold-blooded vertebrate animal to the viruses of warm-blooded animals have detected. The Aujeszky's disease virus titer has reached 3.7 ± 0.07 lg TCDD50/cm3 for 48 hours in the third passage, and the vesicular stomatitis virus titer has reached 5.8 ± 0.12lg TCDD50/cm3 in the third passage for 48 h cultivation. The Aujeszky's disease virus and vesicular stomatitis virus have led to cytomorphological changes in the TF cell culture were similar to those in other cell cultures have occurred. The virus’s replication have destroyed the cells monolayer and have led rounded off cells and separated from the substrate of both reference and TF cell lines.

Conclusions and perspectives of further research: The retrieved TF cell culture, from the pool of internal parenchymal organs of the turtle reddish-brown (Trachemys scripta elegance), is suitable for the study of reproduction of warm-blooded viruses that exhibit pronounced infectious activity in it. This fact, established by us, allows us to make assumptions about the possibility of further use of this culture of cells in the evolutionary research of viruses, in the testing and development, control of prophylactic antiviral drugs, and for other viral studies.

Keywords: cell culture, reptiles, viruses, animals, sensitivity.

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