Bulletin "Veterinary biotechnology"

Veterynarna biotehnologija – Veterinary biotechnology, 2018, 32(1), 245-253 [in Ukrainian]. https://doi.org/10.31073/vet_biotech32(1)-32

SEMKO K.R.e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., PINCHUK N.G.e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

State Scientific Control Institute of Biotechnology and strains (DNKIBSHM)


Introduction. Quality control of sterilization is on of the leading places in modem medical and veterinary practice. The quality of the sterilization process can only be confirmed through various types of control tests indicators, which indicate that all sterilization equipment is operating in the planned standard mode.

The goal of the work. Was to develop a system of biological indicators based on spore-forming microorganisms.

Materials and methods. The purpose of the sterilization method is to active the sterility of the treated materials. A thorough inspections of thermal properies of the most heat-sensitive strains under laboratory conditions in the process of autoclaving and hot-sterilization.

Results of research and discussion. We conducted research on determination of termal properies of spore-forming strains microorganisms Васillus licheniformis G, Васillus stearothermophilus В-718 BKM, Васillus stearothermophilus var.calidolactis c.953, Васillus stearothermophilus ATCC 7953, Васillus cereus (var.mycoides) 537 and Васillus subtilis. Application of the developed «Set of biological indicators» based on specific thermoplasticity of certain strain of microorganisms, determines efficiency of process of sterilization process of medical and veterinary objects.

Conclusions and prospects for further research. 1. The data presented on the results of the determination of the thermal stability of strains Васillus stearothermophilus ATCC 7953, Васillus stearothermophilus В-718 BKM or Васillus licheniformis G showed the possibility use in sets of indicators for steam and dry-sterilization. 2. The uniqueness of the developed «Set of biological indicators» is that they are easy to use, high-quality, modern and available, do not require special laboratory and analytical equipment, compact, do not requare compliance with special safety measures, meet the international requirements regarding their quality. 3. The use of biological indicators gives more informative result of direct control of the effectiveness of sterilization through the death of microorganisms. 4. Sets of biological indicators were tested at state biological factories, laboratories of veterinary medicine and medical institutions.

Keywords: strains, microorganisms, indicator, control, sterilization, biotest.


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