Bulletin "Veterinary biotechnology"

Veterynarna biotehnologija – Veterinary biotechnology, 2018, 32(1), 254-264 [in Ukrainian]. https://doi.org/10.31073/vet_biotech32(1)-33

SIDASHOVA S.e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

ALLC Agricultural company "Petrodolinske", Odessa region


State Scientific-Research Control Institute of Veterinary Medicinal Products and Feed Additives

GRIGORASHEVA I.e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

 LLC "Revival M", Odessa


V.N. Karazin Kharkiv National University


Introduction. Intensification of reproductive function of cows with the high productivity now is the important task of industrial stock-raising. A statistical analysis shows that only 30–75 % all abstracted from the cavity of uterus of cow – donor of embryos suitable to the use, that considerably reduces economic and plant-breeding meaningfulness of method TE.

The goal of the work. We the alternative methodological going offers near preparation of cows – donors to procedure TE, namely: application of complex prophylaxis (therapy + is a prophylaxis) with the use of poly components of probiotic preparation of «Multybacteryn veterinary Bs+La».

Materials and methods. In the complement of this preparation the living symbiotic cultures of Lactobacillus acidophilus, Bacillus subtilis, Bacterium bifidum adolescentis with a concentration no less than 108–9 microbial bodies in 1 cm3. In control cows are a donor were prepared to procedure of the not surgical washing of embryos on a traditional therapeutic chart with the use of antibiotics.

Results of research and discussion. As a result we got from experience cows (n=13), media environment from the cavity of uterus high-performance transparency (92.31 % transparent standards), and in control (n=18) – only 11.11 % standards had sufficient visual transparency at control of in vivo and in vitro. Experimental donor in experience we found out high quality of all got embryos during clear synchronization of staidly development (blastocysts). In the control group of cows – donors in a media environment from the cavity of uterus we found out 32.85 % off-grade embryos and not-fertility ovules.

Conclusions and prospects for further research. The first stages of testing of alternative chart of probiotic defense of mucous cows-donors of embryos testify to perspective of an offer methodical approach on optimization of methods TE and about the necessity of continuation of researches.

Keywords: cow donor of embryos, transplantation, morphology, poly component probiotics, normal micro flora, mucous membranes.


  1. Bugrov, O.D. & Tkachova, І.V. (2014). Znachennya metodu transplantatsiyi embrioniv u systemi selektsiynoyi roboty z maloplodnymy vydamy tvaryn [The value of the embryo transplantation method in the system of breeding work with infertile species of animals]. Naukovo-tekhnichnyy byuletenʹ Instytutu tvarynnytstva NAAN – Scientific and technical bulletin of the Institute of animal science of NAAS, 113, 43–51. [in Ukrainian].
  2. Duvanov, A.V. & Sidashova, S.A. (2013). Transplantatsiya embrionov – al'ternativa importu skota v Ukrainu [Transplantation of embryos is an alternative to importing cattle to Ukraine]. Ekskluzivnye tehnologii – Exclusive technology, 2(23), 50–53. [in Russian].
  3. Zavirjuha, V.I., Homin, S.P., Shalovilo, S.G. & Gamota, A.A. (1987). Profilaktika subklinicheskih endometritov u korov-donorov [Prevention of subclinical endometritis in donor cows]. Tezisy dok. resp. nauch. – prakt. konferencii [Reports theses of the Republican scientific. – practical conference]. (p. 28). Lviv. [in Ukrainian].
  4. Instrukcija po transplantacii embrionov krupnogo rogatogo skota [Instructions for cattle embryo transplantation]. (1987). M.: Gosagroprom. [in Russian].
  5. Korol', S. (2014). Osnovnye zabolevanija KRS na molochnyh fermah Ukrainy. Zabolevanija organov reproduktivnoj sistemy i problemy vosproizvodstva [The main diseases of cattle on dairy farms in Ukraine. Diseases of the reproductive organs and problems of reproduction]. Suchasna veterinarna medicina – Modern veterinary medicine, 2(44), 24–28. [in Russian].
  6. Koshovij, V.P. (2011). Akushers'ko – gіnekologіchnі patologіi u korіv [Obstetric – gynecologic pathologies in cows]. Kharkіv: TOV «Zolotі storіnki». [in Ukrainian].
  7. Madison, V.V. & Madison L.V. (2008). Poliovulyatsiya u korov-donorov – poisk prodolzhayetsya [Polyovulation in donor cows – the search continues]. Naukovo-tekhnichnyy byuletenʹ Instytutu tvarynnytstva NAAN – Scientific and technical bulletin of the Institute of animal science of NAAS, 96, 242–261. [in Russian].
  8. Mel'nik, V.O. & Sіdashova S.O. (2013). Akusherstvo, hinekolohiya i biotekhnolohiya vidtvorennya tvaryn. Konspekt lektsiy [Obstetrics, gynecology and biotechnology of animals reproduction. Summary of lectures.]. Mykolayiv: Mykolayiv State Agrarian University. [in Russian].
  9. Sіdashova, S.O. (2013). Otsinka laktuyuchykh koriv na prydatnistʹ buty donoramy-retsypiyentamy doimplantatsiynykh embrioniv [Estimation of lactation cows for eligibility to be donor-recipients of preimplantation embryos]. Visnyk Poltavsʹkoyi derzhavnoyi ahrarnoyi akademiyi – Bulletin of the Poltava State Agrarian Academy, 2, 61–63. [in Ukrainian].
  10. Sіdashova, S.O., Stahovs'kij, V.F. & Kovtun S.І. (2016). Embrioproduktyvnistʹ koriv-donoriv i funktsionalʹna asymetriya yayechnykiv [Embryo-productivity of donor cows and functional asymmetry of ovaries]. Rozvedennja і genetika tvarin – Breeding and genetics of animals, 51, 247–255. [in Ukrainian].
  11. Sіdashova, S.O. & Gumennij O.G. (2017). Rytmichnistʹ statevykh tsykliv koriv ta rivenʹ prykhovanoyi rannʹoyi embriopatiyi [Rhythmicity of cows' sexual cycles and the level of early latent embryopathy]. Naukovyy visnyk Lʹvivsʹkoho NU veterynarnoyi medytsyny ta biotekhnolohiyi im. S.Z.Hzhytskoho – Scientific herald of the S.Z. Gzhytsky Lviv National University of Veterinary Medicine and Biotechnology, 19(78), 121–127. [in Ukrainian].
  12. Sidashova, S.O. (2017). Metodyka otsinky henetychnoho potentsialu vysokoproduktyvnykh koriv-donoriv embrioniv molochnykh porid pleminnykh stad vitchyznyanykh pidpryyemstv: nauk.-metod. konferentsiyi prof.-vyklad. skladu ta aspirantiv veterynarnoho fakul'tetu ODDAU – Method of estimation of genetic potential of highly productive cows-donor embryos of dairy breeds of breeding herds of domestic enterprises: Mat. sci. method. conference prof. presentation. composition and graduate students of the faculty of veterinary ODDUU [Elektronnyy resurs] – Access mode: http://osau.edu.ua/uk/kontakti. [іn Ukrainian].
  13. Sidashova, S.A. (2012). Effektivnoe vosproizvodstvo: ot diagnoza do stel'nosti Effective reproduction: from diagnosis to pregnancy. Materialy II mezhdunarodnoy nauchno-prakticheskoy konferentsii «Molochnaya imperiya» – Proceedings of the Second International Scientific and Practical Conference Milk empire. (pp. 92–101). Donetsk. [in Russian].
  14. Sіdashova, S.O., Avdoseva, І.K. & Grigorasheva, І.M. (2017). Dosvid zastosuvannya probiotychnoho zakhystu slyzovykh dlya udoskonalennya tekhnolohiyi transplantatsiyi embrioniv VRKH [The experience of using probiotic mucosal protection to improve the technology of transplantation of embryos of cattle]. Naukovo-tekhnich. byul. Instytutu biolohiyi tvaryn i DNDKI vetpreparativ i kormovykh dobavok – Scientific and Technical bullet of Institute of Animal Biology and DNDKI Veterinary and Feed Additives, 17, 116–119. [in Ukrainian].
  15. Rublenko, M.V. & Vlasenko, S.A. (2011). Problemy zabezpechennya zdorov`ya vysokoproduktyvnykh koriv [Problems of providing health to highly productive cows]. Veterinarna medicina: mіzh vіd. temat. nauk. zb. – Veterenary medicine: inter departmental subject scientific collection, 95, 397–400. [in Ukrainian].
  16. Anderson, G.B. (1983). Embryo transfer in domestic animals. Adv.Vet. Sci,., 27, 129–162.
  17. Bai, K. & Huang, І. (2017). Supplemental effects of probiotic Bacillus subtilis FMBJ on growth performance, antioxidant capacity, and meat quality of broiler chickens. Poult. Sci., 96(1), 74–82.
  18. Cutting, S.M. (2011). Bacillus probiotics. Food Microbiol., 28(2), 214–220.
  19. Elliot, L., McMahon, K.J., Gier, H.T., & Marion, G.B. (1968). Uterus of the cow after parturition: Bacterial Content. Am. J. Vet. Res., 29, 77–81.
  20. Kasimanickam, R., Duffield, T.F., Foster, R.A. et al. (2004). Endometrial cytology and ultrasonography for the detection subclinical endometris in postpartum dairy cows. Theriogenology, 62, 9–23.
  21. Kasimanickam, R., Kasimanickam, V., Koziv, V., Lototskiy, V. (2016). Postpartum uterine diseases in dairy cows. Visnyk Bilocerkiv.derzh.agrar. in-tu. – Bulletin of the Bila Tserkva State Agrarian University, 2, 11–16.
  22. Patel, R. & DuPont, H.L. (2015). New approaches for bacteriotherapy: Prebiotics, new generation probiotics, and synbiotics. Clin. Infect. Dis., 60 (2), 108–121.
  23. Selk, G. (2014). Embryo transfer in cattle. Division of Agricultural Sciences and Natural Resources – Oklahoma Cooperation Service, 3158, 4.
  24. Pener, P. (2012). The International Transfer School. Retrieved from: mhtm:file//G:school transfer.mht. [in English].
  25. Transplantation of embryo at dairy cattle. (2017). http://www.aete.eu/ Retrieved from: http://www.aete.eu/index.php/publications-aete/proceedings/151-aete-proceedings-2017/file.
  26. Yong, D. Hassell, T., & Duongan, Y. (2002). Сhronic factors infections: living with unwanted guests. Nature immunology, 3(11), 1026–1032.
  27. Watnik, P. & Kolter, R. (2000). Biofilm, lity of Microbes. J. Bacteriol., 182(10), 2675–2679.

Download full text in PDF