Bulletin "Veterinary biotechnology"

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Veterynarna biotehnologija – Veterinary biotechnology, 2018, 32(1), 254-264 [in Ukrainian]. https://doi.org/10.31073/vet_biotech32(1)-33

SIDASHOVA S., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

ALLC Agricultural company "Petrodolinske", Odessa region

AVDOSEVA I.

State Scientific-Research Control Institute of Veterinary Medicinal Products and Feed Additives

GRIGORASHEVA I., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

 LLC "Revival M", Odessa

STRICSHAK A.

V.N. Karazin Kharkiv National University

PROBIOTIC DEFENSE OF MUCOUS COWS-DONORS AS STAGE OF BIOTECHNOLOGY OF TRANSPLANTATION OF EMBRYOS

Introduction. Intensification of reproductive function of cows with the high productivity now is the important task of industrial stock-raising. A statistical analysis shows that only 30–75 % all abstracted from the cavity of uterus of cow – donor of embryos suitable to the use, that considerably reduces economic and plant-breeding meaningfulness of method TE.

The goal of the work. We the alternative methodological going offers near preparation of cows – donors to procedure TE, namely: application of complex prophylaxis (therapy + is a prophylaxis) with the use of poly components of probiotic preparation of «Multybacteryn veterinary Bs+La».

Materials and methods. In the complement of this preparation the living symbiotic cultures of Lactobacillus acidophilus, Bacillus subtilis, Bacterium bifidum adolescentis with a concentration no less than 108–9 microbial bodies in 1 cm3. In control cows are a donor were prepared to procedure of the not surgical washing of embryos on a traditional therapeutic chart with the use of antibiotics.

Results of research and discussion. As a result we got from experience cows (n=13), media environment from the cavity of uterus high-performance transparency (92.31 % transparent standards), and in control (n=18) – only 11.11 % standards had sufficient visual transparency at control of in vivo and in vitro. Experimental donor in experience we found out high quality of all got embryos during clear synchronization of staidly development (blastocysts). In the control group of cows – donors in a media environment from the cavity of uterus we found out 32.85 % off-grade embryos and not-fertility ovules.

Conclusions and prospects for further research. The first stages of testing of alternative chart of probiotic defense of mucous cows-donors of embryos testify to perspective of an offer methodical approach on optimization of methods TE and about the necessity of continuation of researches.

Keywords: cow donor of embryos, transplantation, morphology, poly component probiotics, normal micro flora, mucous membranes.

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