Bulletin "Veterinary biotechnology"

  • Print

Veterynarna biotehnologija – Veterinary biotechnology, 2018, 32(1), 265-271 [in Ukrainian]. https://doi.org/10.31073/vet_biotech32(1)-34

STEGNIY B., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., MAIBORODA O., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., MEDVID᾽ K., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., MUZYKA D., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., RULA O., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

National scientific center "Institute of Experimental and Clinical Veterinary Medicine"

DYNAMICS OF IMMUNE RESPONSE IN CHICKENS AFTER VACCINATION WITH INACTIVE BIVALENT VACCINE AGAINST AVIAN SALMONELLOSIS

Introduction. Salmonellosis is one of the most common infections. The foodstuffs contaminated with Salmonella is basis of the of people disease.

The goal of the work was to study the the immune response dynamics in chickens after vaccination with inactivated bivalent vaccine against avian salmonellosis.

Materials and methods. For the study were formed 4 groups of chickens for 20 goals each. In order to study the dynamics of the formation of the immune response histological sections were stained immunohistochemically using labeled streptavidin-biotin. The immune response was studied at the level of subpopulations of T- and B-lymphocytes (CD4, IgM, IgG, IgA, and macrophages).

Results of research and discussion. In birds after infection with the strains Salmonella Enteritidis M and Salmonella Typhimurium B, we observed a decrease in the percentage of CD4 cells in the aggregated lymphatic follicles of the blind gut till the 10th day after vaccination, which may indicate transient immunosuppressive properties of the pathogen. An increase in number of IgM- producing cells in vaccinated chickens from 1st and 2nd groups from 7 till 21 day after vaccination has been established. It was noted that level of IgA in chickens from 2nd group on 21 and 51 day after vaccination was 2 times higher than level in 1st group, which indicates the immunostimulating effect of vaccine.

Conclusions and prospects for further research: That this preparation actively affects the cell-mediated immunity, which is confirmed by an intensive formation and accumulation of B-lymphocytes, which produce immunoglobulins.

Keywords: avian salmonellosis, inactivated vaccine, mucosal immunity

REFERENCES

  1. Van Immerseel, F., De Buck, J., De Smet, I., Mast, J., Haesebrouck, F. & Ducatelle, R. (2002). The effect of Vaccination with a Salmonella enteritidis aroA mutant on early cellular responses in caecal lamina propria of newly-hatched chickens. Vaccine, 20, 3034 – 3041.
  2. Revolledo, L. & Ferreira, A.J.P. (2012). Current perspectives in avian salmonellosis: Vaccines and immune mechanisms of protection. Journal of Applied Poultry Research, 21, 418–431.
  3. Shutchenko, P.O. (2012). Vyvchennja mukozal'nogo imunitetu kurchat za eksperymental'nogo zarazhennja virusom infekcijnogo laryngotrahei'tu [Study of mucosal immunity of chickens for experimental infection with the infectious laryngotracheitis virus]. Vet. Medicina – Veterinary medicine, 96, 257–261. [in Ukrainian].
  4. Shutchenko, P.O., Krasnіkov, G.A., Stegnіj, B.T., Stegnіj, A.B., Muzika, D.V., Medvіd', K.O. et al. (2011). Іmunogіstohіmіchne viznachennja іmunnogo stanu kurchat, shheplenih vakcinoju proti visokopatogennogo gripu pticі ta n'jukasls'koї hvorobi [Immunohistochemical determination of the immune status of chickens vaccinated against highly pathogenic avian influenza and Newcastle disease]. Vet. medicina – Veterinary medicine, 95, 315–316. [in Ukrainian].
  5. Caldwell, D.J., Danforth, H.D., Morris, B.C., Ameiss, K.A., & McElroy, A.P. (2004). Participation of the intestinal epithelium and mast cells in local mucosal. Poultry Science, 83, 591–599.
  6. Varmuzova, K., Faldynova, M., Elsheimer-Matulova, M., Sebkova, A., Polansky, O. & Havlickova, H. et al. (2016). Immune protection of chickens conferred by a vaccine consisting of attenuated strains of Salmonella Enteritidis, Typhimurium and Infantis. Veterinary Research, 47, 94.

Download full text in PDF