Bulletin "Veterinary biotechnology"

Veterynarna biotehnologija – Veterinary biotechnology, 2018, 32(1), 277-284 [in Ukrainian]. https://doi.org/10.31073/vet_biotech32(1)-36

STEGNIY M.Yu., STEGNIY B.T.

National scientific center "Institute of Experimental and Clinical Veterinary Medicine"

APPLYING THE CRYOPRESERVATION OF BOVINE LEUCOSIS VIRUS IN BIOTECHNOLOGY OF PRODUCTION LEUKEMIA ANTIGEN

Introduction. At the same time industrial strains serve are the raw materials for manufacturing of diagnostic antigens. In this case the basic regimens to such strains are preservation of their antigenic activity.

The goal of the work. The aim of our work was to study antigen produce activity cell culture (FLK-BLV) during their low temperature 196°C storage National Collection virus strains of infectious animal disease.

Materials and methods. The research object was the replication of bovine leukemia virus in monolayer cell culture (FLK-SBBL) and (FLK -71) strains. Electron microscopy was performed in the samples which were stored from 4 months up to 16 years and 7 months at low (liquid nitrogen temperature –196°C).

Results and discussion. Electron microscopy was performed in the samples which were stored from 4 months up to 16 years and 7 months at low (liquid nitrogen temperatures 196°C) showed the presence of the bovine leukemia viruses on 5 –7 days after thawing of the samples and antigen produce virus activity was not reduced.

Conclusion. Cryopreservation cell culture (FLK-BLV) during their low temperature 196°C storage National Collection virus strains of infectious animal disease antigen produce virus activity was not reduced.

Keywords: leukemia antigen, (FLK-BLV), cryopreservation, ultrastructure.

REFERENCES

  1. Van Regenmortel, M.H.V., Fauquet, C.M., Bishop, D.H.L. et al. (2000). Virus Taxonomy: classification and nomenclature of viruses. Seventh report of the International Committee on Taxonomy of Viruses. San Diego: Academic Press.
  2. Dyakonova, L.P. (2009). Zhivotnaja kletka v kul'ture (Metody i primenenie v biotehnologii) [The animal cell in culture medium. Methods and applications in biotechnology]. – Moscow: "Sputnik+". [in Russian].
  3. Mihaleva, E.A. (1987). Sravnitelnaia harakteristika razlichnyh laboratornyh sistem kultivirovania virusa leikoza krupnogo rogatogo skota [Comparative characteristics of various laboratory systems for the cultivation of bovine leukemia virus]. Extended abstract of candidate’s thesis. Moscow. [in Russian].
  4. Van der Maaten, M.J. & Miller, J.M. (1976). Replication of bovine leukemia virus in monolayer cell culture. Bibl. Haematol., 43, 360–362.
  5. Mamoun, R.Z., Astier, T., Guillermain, B. & Duplan, J.F. (1983). Bovine lymphosarcoma: processing of bovine leukemia virus-coded proteins. Gen.Virol., 64, 12, 2791–2795.
  6. Altaner. C., Ban, J., Zajac, V. et al. (1985). Isolation and characterization of cell clones producing various amounts of bovine leucosis virus. Folia boil., 31, 2, 107–114.
  7. Wekle, B. (1972). Elektronnaia mikroskopia dlia nachinajushhih [Electron microscopy for dummies]. Moscow: Myr. [in Russian].
  8. Stegnіj, B.T., Stegnіj, M.Ju. & Stecenko, V.І. (2011). Ukrainian patent No. UA 60996. Retrieved from http://uapatents.com/2–60996-sposib-kriokonservuvannya-shtamu-pereshheplyuvanikh-klitin-nirki-vivci-flk-sbbl-yakijj-produkueh-virus-lejjkozu-veliko-rogato-khudobi.html.
  9. Stegnii, M. Ju., Stegnii, B.T. & Golcev, A.M. (2012). Kriokonservuvannia shtamu pereshchepliuvanykh klityn nyrky vivtsi FLK-SBBL, yakyi produkuie virus leikozu velykoi rohatoi khudoby [Cryopreservation of the strain of transfused kidney cells FLK-SBBL of sheep witch producing virus of cattle leukemia]. Problemy kriobiologii – Problems of cryobiology, 22, 3., 254. [in Ukrainian].

Download full text in PDF