Veterynarna biotehnologija – Veterinary biotechnology, 2018, 32(1), 308-312 [in Ukrainian]. https://doi.org/10.31073/vet_biotech32(1)-40
CHEPURNA V.A., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
State Agrarian and Engineering University in Podilya
SUPROVУCH T.M., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
State Agrarian and Engineering University in Podilya
VISHCHUR O.I., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
Institute of animal biology of NAAS
KOVALENKO V.L., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
State Scientific Control Institute of Biotechnology and strains (DNKIBSHM)
LEUKOCYTIC AND BIOCHEMICAL PROFILE OF BLOOD COURSES, PATIENTS WITH CLINICAL MASTITIS, FOR THE EFFECT OF LIPOSOMAL DRUGS ON THE BASIS OF ETHYLTHIOSULFANILATE
Introduction. Mastitis cows represent one of the most important problems of livestock breeding. Scientific researches on the use of liposomal drugs, which do not contain antibiotics, contribute to the prevention of relapse of the disease, and to the maximum restoration of milk production, have considerably expanded in the last few years.
The aim of work was to investigate the change of leukocyte biochemical indexes of blood for cowswith the clinical inflammatory process of mammary gland atthe use of the liposomal preparation on the basis of preparation based on ethylthiosulfanilate (ETC) developed by the Institute of Animal Biology of the National Academy of Sciences of the Russian Academy of Sciences.
Materials and methodology of researches. Experience was conducted on twogroups of cows. A control group is healthy animals (n=7), experience groupof cow with the clinical (catarrhal) form of mastitis (n=7) To the cows of the experimental group received intramuscular injection of a new complex liposomal preparation based on ethylthiiosulfanilate (ETS) at a dose of 20 ml per animal for a period of 24 hours at intervals of 24 hours. The whole blood was determined: the number of red blood cells and white blood cells, hemoglobin content, the ratio of individual forms of leukocytes; total protein by biuretovoy reaction.
Results of researches. An amount of leucocytes sick cows had anymore on 55,6 р<0,01), than for healthy animals, that testifies to the expressed leukocytosis. The decline of content of general protein is marked in the serum of blood of sick cows on 5,91 р<0,05). On the 7th day after the beginning of treatment of cowsliposomal preparation on the basis of ethyl thiosulfanilate, is establish reduction of generalamount of leucocytes on 17 and increase of content ofgeneral albumen in the serum of blood on 13,8p<0,001), bycomparison to a level fixed on the first time of experiment. At the same time, the total protein content was 6.9% (p <0.05) higher, compared with its serum content in the control animals. These changes indicate the normalizing effect of this drug.
Conclusion. The use of a liposomal preparation based on ethylthiosulfanilate in the treatment at cows suffering by clinical catarrhal mastitis, provided their recovery and contributed to the increase of total protein and hemoglobin.
Keywords: cow, mastitis, leukocytes, neutrophils, ethylthiosulfanilate.
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