Veterynarna biotehnologija – Veterinary biotechnology, 2018, 32(1), 330-335 [in Ukrainian]. https://doi.org/10.31073/vet_biotech32(1)-44
DEREVIANKO S.V., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., RESHOTKO L.M., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., DMYTRUK O.O., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
Institute of Agricultural Microbiology and Agro-Industrial Production of NAAS
GOLOVKO A.M., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., KATZYMON V.V., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
State Scientific Control Institute of Biotechnology and strains (DNKIBSHM)
IMPROVEMENT OF SYSTEM DIAGNOSTIC AND SPECIFIC PROPHYLAX OF TESCHOVIRUS ENCEPHALOMYELITIS (TESCHEN DISEASE)
Introduction. Recently, the International Committee on Taxonomy of the Virus (ICTV) has made significant changes in the taxonomy and nomenclature of Picornaviridae. The World Organisation for Animal Health (OIE) has proposed new methods for the diagnosis of Teschovirus encephalomyelitis. In this regard, the domestic diagnostic and prophylaxis system of the Teschovirus encephalomyelitis needs to be improved.
The goal of the work. To improve the means of diagnosis and specific prevention of Teschovirus encephalomyelitis in accordance with the recommendations of the World OIE, to develop an inactivated vaccine with high consumer characteristics, and to propose changes to the current "Instructions on measures for the prevention and control of enzootic encephalomyelitis (Teschen disease) of pigs".
Materials and methods of research. Reference and production strains of Teschovirus A, Sapelovirus A fnd Enterovirus G are used in this work. Accumulation of viruses was carried out in a digestive culture of embryonic kidney cells of pigs and kidneys of newborn Syrian hamster. In experiments were used the virus neutralization reaction, immunofluorescence, immunoassay, enzyme-linked immunosorbent assay, polymerase chain reaction with reverse transcription.
Results of research and discussion. On the basis of the new domestic strain Teschovirus A Dniprovsky-34 a set of diagnoses of Teschovirus encephalomyelitis was created in the reaction of neutralization, immunofluorescence, immune peroxidase reaction and in the enzyme-linked immunosorbent assay. For the specific identification of the pathogen, for the first time, a domestic set of diagnosticums was developed in a polymerase chain reaction with reverse transcription. An inactivated vaccine against Teschovirus encephalomyelitis has been created with characteristics that significantly exceed domestic and foreign analogues.
Conclusions and prospects for further research: The means of diagnostics and specific prophylaxis of Teschovirus encephalomyelitis have been improved. According to the requirements of OIE, amendments to the current "Instructions on measures for the prevention and control of enzootic encephalomyelitis (Teschen disease) of pigs", namely: to recommend for the diagnosis of the use of test systems based on immunohistochemistry, enzyme-linked immunosorbent assay and the species identification of the pathogen in polymerase chain reaction with reverse transcription. According to the modern nomenclature and taxonomy of viruses approved ICTV it is necessary to change the name of the pathogen. In addition, according to the recommendations of the OIE, an addition to the name of the illness should be added.
Keywords: Teschovirus, encephalomyelitis of pigs, diagnostik, killed vaccine.
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