Bulletin "Veterinary biotechnology"

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 Veterynarna biotehnologija – Veterinary biotechnology, 2018, 32(1), 336-344 [in Ukrainian]. https://doi.org/10.31073/vet_biotech32(1)-45

DRAGUT S.S., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., STEGNIY B.T., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., BOLOTIN V.I., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., OBUKHOVSKAYA O.V., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., KUTZENKO V.A., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.,  BLIZNETSOV A.G., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., MARCHENKO N.V., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., RAMAZANOVA T.P., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

National scientific center «Institute of Experimental and Clinical Veterinary Medicine»

RAJKO D.U., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

SE RF "Markeevo" of the M.F. Ivanov Institute of livestock breeding of the steppe regions of NAAS

EPIZOOTIC FEATURES AND LABORATORY DIAGNOSTICS OF ASSOCIATION OF OVINE EPIDIDYMITIS (BRUCELLA OVIS) AND CHLAMYDIOSIS INFECTION

The analysis of literature data on the epizootological features and diagnosis of ovine epididymitis (Brucella ovis) and chlamydiosis infection in sheep is carried out. The results of laboratory diagnostics of the association of this infections in the sheep farm "T" of the central-western region of Ukraine are shown. In serological, bacteriological and molecular diagnostic studies 13 samples of blood sera from the sheep number, abortion from the ewes No. 26279, pathological material from the clinically ill clogged ram No. 21116 (sex glands, liver, spleen, kidneys, inguinal lymph nodes) were examined with commercial diagnosticums. Cold CFT on the ovine epididymitis (Brucella ovis) was put according to the method of P.A. Trilenko (1976). Serodiagnosis of chlamydia was carried out in the CFT. In rose-bengal test for brucellosis all animals was seronegative. In ELISA all animals were free from antibodies to the Chlamydophila abortus. In 20% of ewes was detected seropositivity in cold CFT ovine epididymitis (Brucella ovis) and the same number only the sheep showed a dubious reaction. In rams seropositivity in the cold CFT is set at 25%, in AGID test – in 37.5% of animals. In total, 20% of the ewes and 25% of the sheep reacted positively to chlamydia in the CFT. Doubtfully reacting animals for chlamydia in 12.5% of cases were detected only among the rams. A culture of Brucella ovis was isolated from the epididymal gland of the ram No. 21116. The isolate of the pathogen was identified by the culture-morphological, tinctorial and antigenic properties. The diagnosis on the ovine epididymitis (Brucella ovis) is confirmed by the isolation and identification of the pathogen of according to the recommendations of the OIE and current documents of Ukraine. Also, the presence of the genetic material of brucella of the Brucella ovis species and Chlamydia was established in all samples (the pool of abortic organs from the ewe No. 26279, scraping from its surface and appendages of the gonads from ram № 21116) in the PCR. The conclusion is made about the need to control the epizootic situation in the sheep breeding farms of Ukraine of all forms of ownership of the Brucella ovis species and Chlamydia, which cause significant damage to sheep breeding.

Keywords: association, epizootic features, chlamydiosis infection, laboratory diagnostics, ovine epididymitis (brucella ovis).

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