Bulletin "Veterinary biotechnology"

 Veterynarna biotehnologija – Veterinary biotechnology, 2018, 32(1), 394-399 [in Russian]. https://doi.org/10.31073/vet_biotech32(1)-52

LAMAKO U.V., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.NOVIKOVA O.N., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.ZUBOVSKAYA I.V., BELIANKО D.L., DADASHKO S.V.

S.N. Vyshelesky Institute of Experimental Veterinary Medicine, Minsk, Republic of Belarus


Introduction. Gastroenteritis in neonatal calves are the wide spread acute infection pathology in many cattle farms through over the world. The important preventive measures include proper colostrum management, vaccination of pregnancy cows and neonatal calves. There are some problems to determine postimmunisation seroconvershion in paired serum of neonatal calves because of the high level of colostrums antibodies. The effect of the vaccination in neonatal calves depends on many factors such as proper vaccine, revaccination program, level of maternal antibodies, age of calves, influence the vaccine on non-specific resistance and so on.

The goal of the work. The influence of the vaccine for preventive prophylactic of colibacillosis, salmonellosis, klebsiellosis and proteosis in bovine (KSKP) on the specific immune response and phagocytosis in calves up to two month of age was studied.

Materials and methods. The level of antibodies in blood serum was determined in reaction of agglutination (RA). The phagocytosis experiments were perfomed with 24 h culture S. epidermidis. The phagocytic index – the percentage of granulocytes taking part in phagocytosis – the phagocytic number – the average number of microbe cells captured by one granulocyte – and completed phagocytosis- were estimated. The clinical status of calves was estimated too.

Results of the study and discussion. The results showed that vaccine KSKP displayed phagocytosis stimulatory properties and that these properties determined by the activity of Montanide ISA 206. The seroconversion in paired serum of immunization neonatal calves was not found. The clinical status of experimental calves was different. 70% calves not vaccinated with vaccine KSKP or Montanide ISA 206 had diarrhea and loss of weight.

Conclusions and prospects for further research. The facts reported in this article show that calves had high level of colostrum antibodies to vaccine KSKP antigens. Immunization of these calves in early neonatal period with vaccine KSKP resulted in enhancing resistance to nonpathogenic microorganism circulation in dairy farm.

Keywords: vaccine KSKP, specific immunity, non-specific resistance, calves.


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