Bulletin "Veterinary biotechnology"

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Veterynarna biotehnologija – Veterinary biotechnology, 2018, 32(1), 509-513 [in English]. https://doi.org/10.31073/vet_biotech32(1)-68

MANDZJA І.M., VOITA O.S., This email address is being protected from spambots. You need JavaScript enabled to view it.

State Scientific Control Institute of Biotechnology and Stains of Microorganisms, Kiev

OPTIMIZATION OF AVIBACTERIUM PARAGALLINARUM MOLECULAR DIAGNOSTIC

Diagnostic of bird’s contagious illness must be timely and error-free. Sometimes only bacteriologic methods for some bacteria agents’ identification is not enough. One of such bacteria is Avibacterium paragallinarum, that cause contagious poultry disease Infectious coryza. That why the aim of this study was to optimize method for Avibacterium paragallinarum identification by polymerase chain reaction. Selection of temperature and time models give opportunity to make reaction highly sensitivity and specific. Also reaction became economically profitable because of possibility to use combine samples.

Keywords: Infectious coryza, polymerase chain reaction, molecular diagnostics, Avibacterium paragallinarum.

REFERENCES

  1. Yamamoto, R. (1991). Infectious coryza. Diseases of Poultry. (9th ed.). B.W. Calnek, H.J. Barnes, C.W. Beard, W.M. Reid, H.W.J. Yoder, et al. Iowa: State University Press.
  2. Blackall, P.J., Matsumoto, M., Yamamoto, R. (1997). Infectious coryza. Diseases of poultry. (10th ed.). Calnek, B.W., Barnes, H.J., Beard, C.W., McDougald, L.R., Saif, Y.M.(Eds.). Iowa: State University Press.
  3. Blackall, P.J. (1999). Infectious Coryza: Overview of the Disease and New Diagnostic Options. Clinical Microbiology Reviews, 12(4), 627–623.
  4. Akter, S., Ali, M., Das, P.M. & Hossain, M.M. (2013). Isolation and identification of Avibacterium paragallinarum, the causal agent of infectious coryza (IC) from layer chickens in Bangladesh. Journal of the Bangladesh Agricultural University, 11(1), 87–89.
  5. Chen, X, Miflin, J.K, Zhang, P, Blackall, P.J. (1996). Development and application of DNA probes and PCR tests for Haemophilus paragallinarum. Avian Diseases, 40(2), 398–407.

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