Veterynarna biotehnologija – Veterinary biotechnology, 2019,34, 9-14 [in Ukrainian]. https://doi.org/10.31073/vet_biotech34-01

DYBKOVA S.M., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., TIUTIUN S.M., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., RIEZNICHENKO L.S., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., ZHOVNIR O.M., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., ANDRIYASCHUK V.A., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., UKHOVSKA T.M., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

Institute of Veterinary Medicine NAAS

GRUZINA T.G., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

F.D. Ovcharenko Institute of Biocolloidal Chemistry the NAS of Ukraine

ESTIMATION OF GENOTOXICITY AND CYTOTOXICITY OF STIMULATING TISSUE PREPARATION (“STP”) AND “STP” MODIFED BY IRON NANOPARTICLES  

Introduction. Stimulating tissue preparations (“STP”) increase the resistance of the organism of animals. However, the modification of tissue preparations with iron nanoparticles can significantly improve their effectivenes.

The goal of the work was to evaluate genotoxicity and cytotoxicity of stimulating tissue preparation (“STP”) and “STP” modifed by iron nanoparticles.

Materials and methods. Iron nanoparticles were synthesized by the F.D. Ovcharenko Institute of Biocolloidal Chemistry the NAS of Ukraine. For the modification of the STP and STP modifed by iron nanoparticles (“STP+FeNP”) the drug “STP” was mixed with a sterile aqueous dispersion of iron nanoparticles. The final concentration of nanoparticles in the modified preparation was 1.0 mg / ml by metal. The investigations of genotoxicity and cytotoxicity of the “STP” and “STP+FeNP” was performed using eukaryotic cells line ST (testicles of pigs.) The evaluation of cytotoxicity of “STP” and “STP+FeNP” was carried out in vitro by means of evaluation of vitality of cells with the inclusion of the trypan blue. Genotoxicity estimation in vitro for “STP” and “STP+FeNP” has been analyzed by the Comet assay method.

Results of research and discussion. The obtained data indicate an increase in the survival rate of test eukaryotic cells due to the introduction of the “STP” in the culture medium. The evaluation of the cytotoxicity of "STP+FeNP" revealed similar levels of cytotoxicity. Testing of the genotoxicity of the “STP” showed no primary DNA damage in the cells ST line. The "ІDNA" index of test cells treated with the drug ST was 0.076 ± 0.001, which is even below the level primary damages of intact cells for which the "ІDNA"was 0.125 ± 0.001. The evaluation of the genotoxicity of "STP+FeNP" revealed a similar picture.

Conclusions and prospects for further research. It was established that both the STP and "STP+FeNP" are safe in terms of cytotoxicity and genotoxicity. Prospects for further research are the study of the possibility of modifying the preparation “STP” with safe metal nanoparticles of various nature.

Keywords: stimulating tissue preparation“STP”, iron nanoparicles, cytotoxicity, genotoxicity, STP modifed by iron nanoparticles.

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