Bulletin "Veterinary biotechnology"

Veterynarna biotehnologija – Veterinary biotechnology, 2019, 34, 14-20 [in Ukrainian]. https://doi.org/10.31073/vet_biotech34-02

DOBROZHAN I., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

State Scientific and Research Institute of Laboratory Diagnostics and Veterinary and Sanitary Expertise

SHEVCHENKO L., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

National University of Life and Environmental Sciences of Ukraine


Introduction. For prevention of infectious diseases and (or) elimination of poultry diseases that arise under industrial poultry production technology a number of antimicrobial agents are used. This preparations excrete with droppings and accumulate in poultry products and thus are a pollutant that pose risks for environmental and people health.

The goal of the work was to investigate the dynamics of the level of colistin in the chicken litter of the industrial flock during the period of its storage under mesophilic conditions.

Materials and methods. Research was conducted on the basis of State Research Institute of Laboratory Diagnostics and Veterinary and Sanitary Expertise. Subject of research was chicken litter of industrial and replacement flock of high-line white cross-breed of the Kiev oblast. The mass of samples was 200-300 g. Litter samples were taken from flocks which antimicrobial drugs were administered in drinking water. After sampling, the litter samples were stored under mesophilic conditions for 17 months. Content of antibacterial preparations in chicken droppings was determined by routine methods using liquid chromatograph with the mass-spectrometric detector, Waters (USA).

Results of research and discussion. In the litter of the industrial flock chickens, the residual content of colistin was found. Colistin was detected in chickens litter of the industrial flock in concentration ranged from 52,87±0,82 mkg/kg to 77,5±1,14 mkg/kg. In 3 weeks after mesophilic storage, the content of colistin increased and was in range of 78,35±2,56 mkg/kg to 84,87±1,24 mkg/kg. After 3 months of storage, its concentration ranged from 48,34±2,47 mkg/kg to 50,99±2,43 mkg/kg, after 12 months of storage – from 43,40±0,73 mkg/kg to 87,00±1,43 mkg/kg, after 15 months of storage –- from 252,1±20,02 mkg/kg to 286,36±21,43 mkg/kg. After 17 months litter of industrial flock storage the concentration of colistin remained almost the same as after 15 months storage and it was ranged from 207,80±15,71 mkg/kg to 281,00±29,34 mkg/kg. Concentration of colistin in chickens litter of a replacement flock was 91,69±1,60 mkg/kg, after 3 weeks and after 3 months storage colistin was detected in concentrations within 63,10±1,60 mkg/kg and 62,20±0,83 mkg/kg. After 12 months of storage under mesophilic conditions it was showed that its concentration reached the initial level. The storage of litter for 15 months contributed to the accumulation of colistin as evidenced by an increase in its concentration 1,6 times compared with initial data and by 2,2 times after 17 months of storage.

Conclusions and prospects for further research. The using of colistin causes its excretion with litter. Under mesophilic storing conditions of chickens litter the amount of colistin does not decrease, but increases in 12 - 17 months of storage, indicating its exogenous origin.

Keywords: antibiotics, colistin, litter, chickens.


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