Bulletin "Veterinary biotechnology"

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Veterynarna biotehnologija – Veterinary biotechnology, 2019, 34, 32-39 [in Ukrainian]. https://doi.org/10.31073/vet_biotech34-04

YEVTUSHENKO A.V., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

National scientific center «Institute of Experimental and Clinical Veterinary Medicine»

EFFECTIVENESS OF THE DRUG BASED ON CHLOROQUINE FOR CILIOPHOROSIS TREATMENT IN FISH

Introduction. Ciliophorosis is a group of diseases caused by parasitic infusoria, including ichthyophytosis, chylodonelosis, and trichodynosis, which are widespread in Ukraine. The drug “Rybokhin” was designed in the NSC IECVM. Its active substance is chloroquine, the derivative of 4-aminoquinolines. Previous studies have been conducted to confirm low toxicity of chloroquine to fish.

The goal of the work was to study the antiparasitic effectiveness of the drug “Ribokhin” during the spontaneous invasion of fish caused by ciliophorosis causative agents.

Materials and methods. In laboratory experiments the Cyprinus carpio (n=50) spontaneously infested with Chilodonella cyprinid and Trichodina nigra mixed invasion, and Ictalurus punctatus (n=60) spontaneously infested with Ichthyophthirius multifiliis were used. During the experiment fishes received “Ribokhin” individually per os for two days in a daily dose of 2; 5; 10 and 20 mg/kg of body weight (by AI). The effectiveness of “Ribokhin” was estimated under industrial conditions in a dose of 10 mg/kg of fish body weight for two days to treat one year old Ctenopharyngodon idella (n–1500), which were spontaneously infested with Ichthyophthirius multifiliis.

Results of research and discussion. It was confirmed 100% efficacy of “Ribokhin” for chilodonalosis and trichodinosis mixed invasion of carp and ichthyophthiriosis invasion of channel catfish was registered after administration of two doses by 10 mg/kg each (by AI). Daily administration of “Rybokhin” with feed to fish in a daily dose of 10 mg/kg for two days (by AI) provides 100% efficacy in fish ciliophorosis treatment.

Conclusion and prospects for further research. The obtained results indicated that the use of the drug “Ribokhin” by feeding fish in a dose of 10 mg/kg (by AI) for two days provided 100% efficacy for fish ciliophorosis treatment. The preparation can be used in the practice of veterinary medicine for parasitic protozoa diseases control.

Keywords: ciliophorosis, chloroquine, fish, therapeutic dose, antiparasitic efficacy.

REFERENCES

  1. Katjuha, S.M., & Voznjuk, I.O. (2016). Poshyrennja invazijnyh hvorob ryb u vodojmah Rivnenskoi oblasti [Distribution of invasive fish diseases in the reservoirs of the Rivne region]. Veterynarna biotehnologija – Veterinary biotechnology, 28, 94-101 [in Ukrainian].
  2. Petrov, R.V. (2012). Analiz epizootychnoi sytuacii shhodo hvorob prisnovodnoi ryby zaraznoi etiologii v mezhah Ukrainy [Analysis of the epizootic situation regarding freshwater fish diseases of infectious etiology within Ukraine]. Visnyk Sumskogo nacionalnogo agrarnogo universytetu «Veterynarna medycyna» – Bulletin of the Sumy National Agrarian University “Veterinary Medicine”, 7 (31), 119-123 [in Ukrainian].
  3. Stybel, V.V., Berezovskyj, A.V., Dovgij, Ju.Ju. et al. (2016). Invazijni hvoroby ryb: navch. posib. [Invasive fish diseases: Tutorial]. Zhytomyr: Polissja [in Ukrainian].
  4. Nastavlenie po primeneniju tehnicheskih i organicheskih krasitelej (osnovnyh jarko-zelenogo i fioletovogo «K» ot 19 apr. 1971) dlja profilakticheskoj obrabotki ryby v zimovalnyh prudah [Guidelines on the use of technical and organic dyes (the main bright green and purple “K”) for the preventive treatment of fish in wintering ponds from 19 April 1971]. (1971, 19 April). Veterinarnoe zakonodatelstvo – Veterinary legislation, 1, 516-518 [in Russian].
  5. Nastavlenie po primeneniju rastvora formaldegida dlja protivoparazitarnoj obrabotki ryb v rybovodnyh zimovalnyh kompleksah ot 28 fevralja 1984 g. [Guidelines on the use of formaldehyde solution for antiparasitic treatment of fish in fish-breeding wintering complexes].(1984, 28 February). Veterinarnoe zakonodatelstvo – Veterinary legislation, 4, 582-587 [in Russian].
  6. Krafts, K., Hempelmann, E., & Skórska-Stania, A. (2012). From methylene blue to chloroquine: a brief review of the development of an antimalarial therapy. Parasitol Res., 111(1), 1-6. doi: 10.1007/s00436-012-2886-x.
  7. Vaziri, A., & Warburton, B. (1994). Slow release of chloroquine phosphate from multiple taste-masked W/O/W multiple emulsions". Journal of Microencapsulation. 11 (6), 641-8. doi: 10.3109/02652049409051114.
  8. Hemdal, J. (2013). Aquarium Fish: Chloroquine: A «New» Drug for Treating Fish Diseases. Advanced Aquafish, Vol. XII, Issue I. URL: https://www.advancedaquarist.com/2013/2/fish.
  9. Byhovskaja-Pavlovskaja, I.E. (1985). Parazity ryb: Rukovodstvo k izucheniju [Fish Parasites: A Guide to Learning]. L.: Nauka [in Russian].
  10. Markevich, A.P. (1951). Parazitofauna presnovodnyh ryb USSR [Parasitofauna of freshwater fish of the Ukrainian SSR]. K.: Izd-vo AN USSR [in Russian].
  11. Demidov, N.V., & Berezkina, S.V. (1986). Metodicheskie rekomendacii po ocenke angelmintikov v veterinarii [Methodical recommendations on the assessment of angelmintik in veterinary medicine]. M.: VASHNIL [in Russian].
  12. European convention for the protection of vertebrate animals used for experimental and other scientific purposes. Council Directive 86/609/EEC of 24 November 1986 on the approximation of laws, regulations and administrative provisions of the Member States regarding the protection of animals used for experimental and other scientific purposes. (1986). Official Journal of the European Communities, L. 358, 1-29.

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