Bulletin "Veterinary biotechnology"

Veterynarna biotehnologija – Veterinary biotechnology, 2019, 34, 50-58 [in Ukrainian]. https://doi.org/10.31073/vet_biotech34-06

ZHOVNIR A.M., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., ANDRIIASHCHUK V.A., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., UKHOVSKA T.M., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., TІUTІUN S.M., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., MINTSUK E.P., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

Institute of Veterinary Medicine NAAS


Introduction. Vaccinology is one of the most important areas of medicine. This science is engaged in the development of vaccines, the study of the immune response and the influence of the vaccine components on the organism. Therefore, the development of new and improving existed specific prevention means has always been its priority.

The goal of the work was to compare the hematological characteristics of the vaccinated animals before and after the usage of vaccines manufactured with traditional and advanced technology (nanoparticles of metals).

Materials and methods. 4 groups of rabbits were formed for this experiment: one control and 3 experimental ones. Blood samples were taken before vaccination, 7 and 14 days after first inoculation, 7, 14, and 21 days after the re-inoculation. Vaccination of animals was carried out in accordance with the provisional guidelines for the usage of experimental vaccines. Hematologic studies were performed according to commonly accepted methods.

Results of research and discussion. The red blood cells quantity analysis in experimental and control groups of rabbits showed that in the experimental group of animals # 3 (rabbits vaccinated with the Velshisan vaccine) this indicator increased on the 7 day of the experiment by 30% compared with the initial data and by 14% compared with the control group. On the 21st day of the experiment red blood cells quantity continued to increase by about 1.5 times compared to the initial data. This indicates that the components of the vaccine have positive influence on hematopoiesis of vaccinated rabbits. According to the analysis of the total number of leukocytes, it is evident that on 7 day after the first vaccination, leukocyte indicators of the rabbits of the experimental groups increased from 27% to 37%. These indicators almost did not change until the end of the experiment because of the activation of the cell-mediated immunity.

Conclusions and prospects for further research. 1. The rabbits vaccinated with a “Velshisan” prototype vaccine showed activation of the erythropoiesis on the 7 day after re-inoculation. The red blood cells quantity increased by 25.0%. 2. The total number of leukocytes in the animals of experimental groups was higher by 12.0% and 18,0% (р<0.01) compared to this indicator in rabbits of the control group. 3. The most stable indicators were in rabbits vaccinated with “Velshisan+АuNP” because the hemoglobin level slightly varied in animals of control and experimental groups (from 128,5±0,35 to 142.0±0.70 g/l). In the future we will study effect of various concentrations of AuNP on cellular and humoral immunity, since gold nanoparticles have active immune-stimulating properties, which will improve the quality and effectiveness of the vaccine preparations.


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