Bulletin "Veterinary biotechnology"

Veterynarna biotehnologija – Veterinary biotechnology, 2019, 34, 59-66 [in Ukrainian]. https://doi.org/10.31073/vet_biotech34-07

KASJANENKO O.I., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., GUSEV V.A., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., KASJANENKO S.M., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., NAGORNA L.V., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., PUNKO I.V., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

Sumy National Agrarian University


Introduction. Campylobacteriosis is considered to be the most frequently reported cause of zoonotic illness globally, with poultry being the main source of infection. Reducing the colonization level of Campylobacter spp. in broilers entering the processing unit could make an effective contribution at reducing the incidence of zoonotic transmission of this pathogen.

The goal of the work was to study of the spread of Campylobacter spp. among slaughter poultry, based on the data obtained, provide a scientifically sound approach to the control of campylobacteriosis.

Materials and methods. We have used ISO 10272-1:2017 Microbiology of the food chain -- Horizontal method for detection and enumeration of Campylobacter spp. – Part 1: Detection method. Results of research and discussion. In the first stage, we have isolated the Сampylobacter spp. from the broiler cecums content of the slaughter poultry. The level of infection by C.jejuni was 8.6-21.8%, and after feed withdrawal – 4.8-14.6%. Infection rates of the cecum content with C.coli were 2.9-3.8% and 2.6% respectively. The biochemical identification of Campylobacter spp. colonies was performed according to ISO 10272-2 protocol. We have differentiated isolates of Campylobacter by the biochemical test. As a result, 48 strains of C.jejuni and 8 strains of C.coli were identified. We have also isolated Campylobacter spp. from swabs of cages in which poultry was transported.

Conclusions and prospects for further research. Measures of control of campylobacteriosis in slaughtered poultry are: feed withdrawal; washing and disinfection of premises, where poultry is kept before slaughter, and transportation cages as well.

Keywords: Campylobacters, products of the poultry, isolation, contamination.


  1. Fotina, T.I., Berezovskyj, A.V., & Kasjanenko O.I. (2010). Kampilobakterioz ptyci. [Campylobacteriosis of poultry]. Sumy: Sumskyj nacionalnyj agrarnyj universytet [in Ukrainian].
  2. Kasjanenko, O.I., Gladchenko, S.M., & Bezruk, R.V. (2016). Strategii kontrolju harchovyh zoonoziv v ptahivnyctvi [Strategies of the control of food zoonosis in poultry farming]. Veterynarna medycyna: Mizhvid. temat. nauk. zb. – Veterinary Medicine: Inter-Departmental subject scientific collection, 102, 155-157 [in Ukrainian].
  3. Kasjanenko, O.Y., Fotyna, T.Y., & Nagorna L.V. (2017) Analyz praktycheskyh aspektv kontrolja vozbudytelej pyshhevih zoonozov pry virashhyvanyja ptyci [Analysis of practical aspects of control of food zoonotic agents during bird breeding]. Uchenie zapysky – Scientific notes, 53, V. 1, 55-58.
  4. Fotina, T.I., Dvorska, Ju.Je., & Kasjanenko O.I. Metodychni vkazivky z veterynarno-sanitarnogo kontrolju mjasa ptyci ta jajceproduktiv na najavnist zbudnykiv zoonoziv (Sampylobacter, E.coli O157, Enterobacteriacae, Listeria, Salmonella, Pseudomonas, Yersinia). Vidbir prob [Methodical instructions on veterinary and sanitary control of poultry meat and egg products for the presence of zoonotic agents (Campylobacter, E. coli O157, Enterobacteriacae, Listeria, Salmonella, Pseudomonas, Yersinia). Sampling]. Sumy: VVP «Mrija» TOV [in Ukrainian].
  5. Verbyckyj, P.I., Berezovskyj, A.V., & Fotina T.I. (2011). Metodychni rekomendacii z vydilennja mikroorganizmiv rodu Sampylobacter z produkcii ptahivnyctva ta porjadku ii veterynarno-sanitarnoi' ekspertyzy [Guidelines on the isolation of the microorganisms of the genus Smylobacter from poultry production and the procedure for its veterinary and sanitary examination]. Sumy: VVP «Mrija» TOV [in Ukrainian].
  6. Fotina T.I., Kasjanenko O.I., Fotina G.A. (2008). Pozhyvne seredovyshhe dlja kampilobakterij [Nutrient medium for Campylobacteria]. Patent of Ukraine for the utility model No. 36641. A 200712445. (2008.11) [in Ukrainian].
  7. Kasjanenko O.I., Gladchenko S.M., Berezovskyj A.V. (2016). Sposib selektyvnoi izoljacii mikroorganizmiv rodu Campylobacter iz produktiv tvarynnogo pohodzhennja i objektiv zovnishn'ogo seredovyshha [Method of selective isolation of microorganisms of the genus Campylobacter from products of animal origin and objects of the environment]. Patent of Ukraine for the utility model No. U 201510861. (2016.06) [in Ukrainian].
  8. Seredovyshhe pozhyvne shhilne dlja kul'tyvuvannja kampilobakterij [The environment is nutritious for cultivating Campylobacter]. (2010). TU U 24.4-14332579-056:2010 of April 16, 2010. – K.: Ukrmetrteststandart Ukrainy [in Ukrainian].
  9. Selektyvna domishka do pozhyvnyh seredovyshh dlja izoljacii' Sampylobacter spp. [Selective admixture for nutrient medium for the isolation of Campylobacter spp]. (2012). TU U 20.5-14332579-056:2012 of October 17, 2012. – K.: Ukrmetrteststandart Ukrainy [in Ukrainian].

Download full text in PDF