Bulletin "Veterinary biotechnology"

Veterynarna biotehnologija – Veterinary biotechnology, 2019, 34, 136-140 [in Ukrainian]. https://doi.org/10.31073/vet_biotech34-16

SAPACHOVA M., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., PISHCHANSKYI O., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., SUSHKO M., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., USACHENKO N., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it. MEZHENSKYI A., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

State Scientific and Research Institute of Laboratory Diagnostics and Veterinary and Sanitary Expertise

RESULTS OF LABORATORY CONTROL OF SOME INFECTIOUS ANIMAL DISEASES IN UKRAINE IN 2018 USING MOLECULAR-GENETIC METHODS

Introduction. Polymerase chain reaction (PCR) is a highly specific and highly sensitive method that is used for the diagnosis of infectious diseases with peracute, acute, chronic and latent courses. The duration of the test is 5-6 hours that characterize this method as fast one. An important advantage of PCR, in comparison with others methods, is the possibility to type and determine a pathogenicity of the agent in laboratories with biosafety level 2. Therefore, the inclusion of molecular genetics methods for conducting tests in a framework of a state control over infectious animal diseases is reasonable.

The goal of the work was analysis of results of molecular genetics tests on African swine fever (ASF), Classical swine fever (CSF), Avian influenza (AI), Newcastle disease (ND) and Lumpy skin disease (LSD) in 2018.

Materials and methods. The research was conducted in the framework of state control of infectious animal diseases. An analysis and summarizing of the results of tests by PCR, obtained in State Scientific and Research institute of Laboratory Diagnostics and Veterinary and Sanitary Expertise the regional state laboratories in 2018 were carried out. Results of research and discussion. 54,799 samples of biological material were tested by PCR, 48 270 samples for ASF (45,288 from domestic pigs and 2988 from wild boars), 642 - on CSF (84 from domestic pigs and 558 from wild boars), 2641 – on AI, 131 – on ND and 3115 - on LSD. As a result of the tests, 267 ASF positive samples in domestic pigs and 71 positive samples in wild boars were detected. The results of CSF, AI, ND and LSD were negative.

Conclusions and prospects of further research. 1. Molecular genetic tests in the system of epizootic monitoring play role of express method, which allows to respond and to control situation promptly and in time. 2. According to the results of the tests in 2018, there was not registered circulation of ND, CSF, AI, and LSD pathogens. 3. The results of the test on ASF indicate on continuation of ASF virus circulation in Ukraine.

Keywords: monitoring, molecular genetic studies, diagnostics.

REFERENCES

  1. Manual of Diagnostic Tests and Vaccines for Terrestrial Animals 2018 [Electronic resource]. oie.int. Retrived from: http://www.oie.int/standard-setting/terrestrial-manual/access-online.
  2. Pryskoka, V.A. (2014). Diahnostyka infektsiinykh zakhvoriuvan tvaryn: teoriia i praktyka [Diagnostics of animal infectious diseases: theory and practice]. Kyiv [in Ukrainian].
  3. Glik B., & Pasternak Dzh. (2002). Molekuljarnaja biotehnologija. Principy i primenenie [Molecular biotechnology. Principles and application]. M.: Mir [in Russian].
  4. Ericka A. Early, Rapid and Sensitive Veterinary Molecular Diagnostics - Real Time PCR Applications: book/ A. Ericka, B. Sandor, A. Diallo, J. Crowther, G. Viljoen. – Springer, 2010. – 310 p. [in English].
  5. Stehnii, B.T., & Herilovych, A.P. (2006). Polimerazna lantsiuhova reaktsiia u praktytsi veterynarnoi medytsyny. Naukovo-metodychnyi posibnyk [Polimerase chain reaction in practice of veterinary medicine. Methodological reference book]. Kharkiv [in Ukrainian].

Download full text in PDF