Veterynarna biotehnologija – Veterinary biotechnology, 2019, 35, 9-14 [in Ukrainian]. https://doi.org/10.31073/vet_biotech35-01
Odessa Scientific Research Station NSC “IECVM”
National Scientific Center "Institute of Experimental and Clinical Veterinary Medicine"
MORPHOLOGICAL CHANGES IN THE BLOOD OF SHEEP vaccinated AGAINST INFECTIVE AGALACTIA OF SHEEP AND GOATs
Introduction. Improvement of the existing and development of new means of specific prevention from infectious diseases is important task of the veterinary science. The success in prevention of infectious agalactia of sheep and goats and its elimination depends mostly on the vaccine quality.
The goal of the work. To determine the morphological changes in the blood of sheep vaccinated with an inactivated vaccine against infectious agalactia of sheep and goats.
Materials and methods. According to the principle of analogues, two groups of sheep (n = 15) were formed with the same conditions of feeding, care and keeping. Control of the vaccine efficacy was performed according to the morphological indicators of blood before vaccination, on the 14th, 21st and 30th days after vaccination, with revaccination on the 60th day.
Results of research and discussion. Already on the 14th day, an inactivated vaccine against infectious agalactia of sheep and goats in the experimental group of animals led to an increase in hemoglobin by 6.3% (p<0.05) compared to control, and by 21st day this indicator increased by 8.4%. The number of erythrocytes increased by 22.5% and 22.6%, respectively, and by 18.3% after revaccination. The stimulating effect of inactivated vaccine on myeloid elements of the blood has been established, as the total number of leukocytes had a positive tendency to increase by 19.8%. Synthesis of heparin and histamine is the main function of basophils in the leukogram. The greatest increase in basophils by 40% was recorded on the 30th day after vaccination and by 60% after revaccination. Eosinophil growth by 47.6% and 79% was also reported on the 30th and 60th days respectively. The number of lymphocytes after vaccination increased by 5.8%, and after revaccination - by 11.2%. Thus, a positive correlation of leukocyte content and an absolute lymphocyte count in the experimental group of animals indicates the activation of cellular and humoral immunity.
Conclusions and prospects for further research. The increase in the of hemoglobin and erythrocytes level and the absolute lymphocytes count in the peripheral blood of sheep vaccinated with an inactivated vaccine indicated activation of the animal's immune cellular status.
Keywords: sheep, infectious agalactia, vaccine, blood morphology.