Bulletin "Veterinary biotechnology"

Veterynarna biotehnologija – Veterinary biotechnology, 2019, 35, 80-89 [in Russian]. https://doi.org/10.31073/vet_biotech35-10

KRASOCHKO P.A., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., KRASOCHKO P.P., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., KURDEKO A.P., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

Vitebsk State Academy of Veterinary Medicine, Republic of Belarus

ZVAVORONOK S.V., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., DOVIDOV V.V., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., ARABIE A.A., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

Belarusian State Medical University

BORISOVETS D.S., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., PROKOPENKOVA T.M., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

S.N. Vishelesky Institute of Experimental Veterinary Medicine, Republic of Belarus

NYCHYK S.A., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

Institute of Veterinary Medicine NAAS, Ukraine

STUDY OF THE ROLE OF ANIMALS AS THE RESIDUES OF THE HUMAN HEPATITIS VIRUS

Introduction. Interest to the problem of viral hepatitis E has grown in the last decade. In studies conducted in different countries, the hepatitis E virus (HEV) was detected in animals (wild boars, pigs, birds, wild rats, etc.), and the role in the occurrence of acute hepatitis E in humans was demonstrated. Animals are involved in the circulation of the HEV in nature, therefore, hepatitis E is a zoonosis. In countries where hepatitis E was detected and serological studies were conducted among various animal species, it was shown that most number of antibodies were registered in pigs, rabbits, birds, cattle.

The goal of the work was to conduct studies on the establishment of the circulation of the HEV in rabbits, domestic and wild pigs in the Republic of Belarus.

Materials and methods. To determine the serological markers of HEV infection an enzyme immunoassay was used for anti-VGE detection using the Vector-Best Cytogenesis-E test kit (Novosibirsk). We developed a test kit for detecting antibodies to HEV in the blood serum of pigs and rabbits using the indirect ELISA, where recombinant ORF2 and ORF3 antigens were used. Determination of the IgG-antibodies using conjugate consisted of horseradish peroxidase with solution of Staphylococcus aureus protein. In this study 124 samples of rabbit blood serum, 987 samples of blood serum obtained from domestic pigs and 11 from wild boars were analyzed.

Results of research and discussion. Revealed results showed that in domestic pigs from various pig farms antibodies were detected in 26.7% of the examined pigs and in 20% of the examined rabbits.

Conclusions and prospects for further research. The circulation of the HEV among some animals in the Republic of Belarus was proved, its approximate scales were determined.

Keywords: hepatitis E virus, pigs, rabbits, antibodies.

REFERENCES

  1. Lvov, D.K. (Eds.). (2013). Rukovodstvo po virusologii: Virusnye i virusnye infekcii cheloveka i zhivotnyh [Virology manual: viral infections in human and animals]. Moscow: MIA [in Russian].
  2. Zhavoronok, S.V., Arabey, A.A., Yagovdik-Telezhnaya, Ye.N. et al. (2017). Avtohtonnyj gepatit E (ehpidemiologiya v gruppah riska, diagnostika, klinika), rasprostranenie u zhivotnyh v Respublike Belarus [Autochthonous hepatitis E (epidemiology in risk groups, diagnosis, clinic), spread among animals in the Republic of Belarus]. Infekciya i immunitet ‒ Infection and immunity, 5, 22-27 [in Russian].
  3. Geng, J., Fu, H., Wang, L., Bu, Q. & Liu, P. (2011). Phylogenetic analysis of the full genome of rabbit hepatitis E virus (rbHEV) and molecular biologic study on the possibility of cross species transmission of rbHEV. Infection, Genetics and Evolution, 11, 2020-2025.
  4. Arabej, A.A., Marchuk, S.I., Makarevich, Zh.A., Zhavoronok, S.V., Kyuregyan, K.K., Mihajlov, M.I. et al. (2017). Yavlyayutsya li domashniye zhivotnyye rezervuarom virusnogo gepatita Ye u cheloveka? Rezultaty molekulyarno-geneticheskikh issledovaniy s ispolzovaniyem adaptirovannogo metoda PTSR-analiza [Are pets a reservoir of viral hepatitis E in humans? The results of molecular genetic studies with an adapted method of PCR analysis]. Laboratornaya diagnostika. Vostochnaya Evropa ‒ Laboratory diagnosis. Eastern Europe, 3(6), 343-351 [in Russian].
  5. Tyagi, S., Surjit, M. & Lal, S.K. (2005). The 41-amino-acid C-terminal region of the hepatitis E virus ORF3 protein interacts with bikunin, a kunitz-type serine protease inhibitor. Journal of Virology, 79(18), 12081-12087.
  6. Meng, X.J., Shivaprasad, H.L. & Payne, C. (2008). Hepatitis E virus infections. Diseases of poultry (12-th. ed.). Saif, Y.M., Fadly, A.M., Glisson, J.R., McDougald, L.R., Nolan, L.K. (Eds.). Ames: Blackwell Publishing Press.
  7. Pavio, N., Meng, X.J. & Renou, C. (2010). Zoonotic hepatitis E: animal reservoirs and emerging risks. Veterinary Research, 41, 46.
  8. Meng, X.J., Purcell, R.H., Halbur, P.G., Lehman, J.R., Webb, D.M., Tsareva, T.S. et al. (1997). A novel virus in swine is closely related to the human hepatitis E virus. Proceedings of the National Academy of Sciences, 94, 9860-9865.
  9. Sonoda, H., Abe, M., Sugimoto, T., Sato, Y., Bando, M., Fukui, E. et al. (2004). Prevalence of hepatitis E virus (HEV) Infection in wild boars and deer and genetic identification of a genotype 3 HEV from a boar in Japan. Journal of Clinical Microbiology, 42, 5371-5374.
  10. Haqshenas, G., Shivaprasad, H.L., Woolcock, P.R., Read, D.H. & Meng, X.J. (2001). Genetic identification and characterization of a novel virus related to human hepatitis E virus from chickens with hepatitis-splenomegaly syndrome in the United States. Journal of General Virology, 82, 2449-2462.
  11. Zhao, C., Ma, Z., Harrison, T.J., Feng, R., Zhang, C., Qiao, Z. et al. (2009). A novel genotype of hepatitis E virus prevalent among farmed rabbits in China. Journal of Medical Virology, 81, 1371-1379.
  12. Johne, R., Plenge-Bonig, A., Hess, M., Ulrich, R.G., Reetz, J. & Schielke, A. (2010). Detection of a novel hepatitis E-like virus in faeces of wild rats using a nested broad-spectrum RT-PCR. Journal of General Virology, 91, 750-758.

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