Bulletin "Veterinary biotechnology"

Veterynarna biotehnologija – Veterinary biotechnology, 2019, 35, 90-99 [in Russian]. https://doi.org/10.31073/vet_biotech35-11

KRASOCHKO P.А., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., YAROMCHYK Y.P., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., KRASOCHKO P.P., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., KRASOCHKO V.P., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., SINITSA N.V., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

Vitebsk State Academy of Veterinary Medicine, Republic of Belarus

SHASHKOVA Y.А., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

OJCM "BelVitunipharm"

TARASOV O.A., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

Institute of Veterinary Medicine NAAS, Ukraine

ADJUVANTAS IN CONSTRUCTION OF MIXED VACCINES AGAINST INFECTIOUS ENTERITES OF CALVES

Introduction. In an etiology of gastrointestinal tract diseases of calves the main role is played by viruses of infectious rhinotracheitis, diarrhea, rotavirus and coronavirus infection, escherichiosis and salmonellosis. The main method of their control means of specific prevention. Modern methods of production of the inactivated virus and bacterial vaccines include use of adjuvants to increase their immunogenicity. A number of adjuvants – mineral (an aluminum hydroxide, potassium alum), oil (an incomplete Freund's adjuvant, emulcigen, montanida, Marcala), natural polysaccharides is known (bacterial lipopolysaccharides, the activated cellulose) are known. In this regard selection of optimum adjuvant when designing vaccines is a current problem in biotechnology.

The goal of the work – to carry out selection of optimum adjuvants when designing mixed vaccines against a viral and bacterial gastroenteritis of young cattle.

Materials and methods. Experimental works were carried out to JSC Belvitunifarm and UO "Vitebsk State Academy of Veterinary Medicine". For work were used the vaccine against infectious rhinotracheitis, diarrhea, rotavirus and coronavirus infection, salmonellosis and escherichiosis with various adjuvants, used in the conditions of JSC Belvitunifarm. Experimental samples of vaccines with various adjuvants in test dose were introduced to laboratory animals and the results of their immunogenicity were estimated in blood sera.

Results of a research and discussion. On the basis of the analysis of the received results of serological researches of laboratory animals blood sera, which were immunized with experimental sample of mixed vaccines against infectious gastroenteritis of calves, was established that all adjuvants had a positive impact on biosynthesis of specific antibodies. At the same time a vaccinated animals antiserum capacity increased by 4-7 log2.

Conclusions and prospects of further researches. When choosing optimum adjuvant in the designed vaccines against gastroenteritis of an infectious etiology of calves, more pronounced stimulation of a humoral immune response were received when using oil IZA-15 and IZA-25 adjuvant.

Keywords: vaccine, blood serum, antibodies, adjuvants.

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