Bulletin "Veterinary biotechnology"

Veterynarna biotehnologija – Veterinary biotechnology, 2019, 35, 110-116 [in Russian]. https://doi.org/10.31073/vet_biotech35-13

PRITYCHENKO A.V., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., KRASOCHKO P.A., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., PRITYCHENKO A.N., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., PONASKOV M.A., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., SHAGAKO N.M., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., VELEVA E.R., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

Vitebsk State Academy of Veterinary Medicine, Republic of Belarus

HUDZ N.V., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

Institute of Veterinary Medicine NAAS, Ukraine

AMINO ACID COMPOSITION OF THE FILTRATE OF THE CULTURE FLUID OF LACTIC ACID BACTERIA

Introduction. Improvement of methods to increase animal safety is an urgent task of industrial animal husbandry. Creation and application of new medical probiotic preparations is a perspective direction, allowing to improve schemes and methods of their application and to provide manufacture of ecologically pure products.

The goal of the work was to study the composition of free amino acids in products of metabolism of mono- and mixed cultures of lactic acid bacteria.

Materials and methods. The composition of free amino acids was studied by capillary electrophoresis in products of monocultures metabolism of different strains of Lactobacillus acidophilus and Streptococcus salivarius, as well as mixed cultures obtained by their joint cultivation in a dairy substrate.

Results of research and discussion. It has been established that cultures of strains of lactic acid bacteria have a pronounced biosynthetic activity. In the process of cultivation of microorganisms in the dairy substrate at the optimum temperature, deep hydrolysis of milk proteins occurs, which is expressed in a manifold increase in the amount of free amino acids compared to their content in the substrate. The qualitative and quantitative composition of free amino acids in the products of the metabolism of monocultures of various strains of Lactobacillus acidophilus and Streptococcus salivarius, as well as in mixed cultures, obtained by culturing them together in a dairy substrate, was studied. The metabolic products of mono- and mixed cultures, obtained after the separation of lactic acid bacteria-producers, can be successfully used as a highly active environmentally friendly substance when designing probiotic preparations, or as an independent therapeutic and prophylactic agent.

Conclusions and prospects for further research: the obtained results indicate a high biosynthetic activity of the culture of Lactobacillus acidophilus and Streptococcus salivarius strains. Products of metabolism of mono- and mixed cultures obtained after separation of lactic acid-producing bacteria can be successfully used as a highly active environmentally safe substance in the design of probiotic drugs, or as an independent therapeutic and prophylactic agent.

Keywords: lactic acid bacteria, metabolic products, amino acids, probiotics.

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