Bulletin "Veterinary biotechnology"

Veterynarna biotehnologija – Veterinary biotechnology, 2019, 35, 116-128 [in Ukrainian]. https://doi.org/10.31073/vet_biotech35-14

RIEZNICHENKO L.S., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., DYBKOVA S.M., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.,

F.D. Ovcharenko Institute of Biocolloidal Chemistry NAS Ukraine

Institute of Veterinary Medicine NAAS

DOROSHENKO A.M., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

Bogomolets National Medical University


Introduction. Among the vital microelements which deficiency leads to the greatest damage in livestock, especially in pig breeding, the leading role belongs to iron. Iron deficiency can lead to loss of 20–30% of the suckling piglets in the first weeks. So, search for new remedies effective in prevention of iron deficiency and the treatment of iron deficiency anemia is an urgent task.

The goal of the work was to determine in vivo the iron nanoparticles effectiveness in the treatment of iron deficiency anemia under their oral administration to experimental animals.

Materials and methods. Synthesized biosafe iron nanoparticles were used in this study. Their antianemic properties were studied on the model of iron deficiency anemia of female Wistar rats. Antianemic effectiveness was estimated under the 10-days treatment course of nanoparticles oral administration in the dose of 12 mg/kg. Iron(III)-hydroxide polymaltose complex (IPC) was used as comparison drug. Blood parameters (hemoglobin and iron concentrations, transferrin saturation percentage) were analysed using standard biochemical kits. The state of microflora in lower part of rats’ gastrointestinal tract has been determined according to the standard microbiological protocols.

Results of research and discussion. It was determined high antianemic activity of the studied iron nanoparticles in comparison with IPC during the experimental 10-days treatment course. Normalization of all estimated marker blood parameters was observed. Together with blood parameters it was established normalization of quantitative parameters of protective and transient intestinal microorganisms in lower part of rats’ gastrointestinal tract up to a level of healthy animals. During in vitro studies using Lactobacillus acidophilus strain it was confirmed the stimulating effect of iron nanoparticles on the growth activity of probiotic bacteria.

Conclusions and prospects for further research. Based on the analysis of complex parameters it was revealed high effectiveness of the investigated iron nanoparticles as a potential new means for prevention and treatment of iron deficiency anemia in animals. The prospects for further research are development of metal-containing probiotics as a new class of immunobiological preparations.

Keywords: iron nanoparticles, iron deficiency, dysbiosis, normalization, antianemic activity.


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