Bulletin "Veterinary biotechnology"

Veterynarna biotehnologija – Veterinary biotechnology, 2019, 35, 9-21 [in Ukrainian]. https://doi.org/10.31073/vet_biotech36-01

BOIKO P.K., е-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., BOIKO O.P., е-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., NYCHYK S.A., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

Institute of Veterinary Medicine NAAS

SYTNIK V.A., е-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., MAZUR V.M., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

National University of Life and Environmental Sciences of Ukraine

CATTLE INFECTION CAUSED BY ATYPICAL MYCOBACTERIA IN A SEPARATED HERD

Introduction. The main method of intravital diagnosis of tuberculosis is the allergic method. Persistence of atypical mycobacteria in the environment leads to the appearance of paraallergic reactions to tuberculin in animals. In such cases, the methodology of complex study for tuberculosis is simultaneous test, which allows to establish the diagnosis for tuberculosis reliably.

The goal of the work. To study the nature of para allergic (false positive) reactions to the tuberculin skin test (with tuberculin purified protein derivative (PPD) in cows of the tuberculosis free farm of Volyn' oblast.

Materials and methods. The epizootological, allergic, clinical, pathological, histological, bacteriological and biological research methods were used.

Results of research and discussion. The results of the study showed that the false-positive tuberculin skin tests or para allergic reactions on PPD in cows was the extended asymptomatic infectious process, caused by atypical mycobacteria. These thesis were confirmed bacteriologically. M. fortuitum and M. scrofulaceum were identified from the lymph nodes of animals with of positive tuberculin skin test.

Based on the results of the study, a comprehensive approach system of measures was developed to prevent slaughtering of cows and the economic losses.

Conclusions and prospects for further research:

1. The false positive reactions to the tuberculin skin test in cows were induced by atypical acid-resistant mycobacteria.

2. The regular use of Atypical Mycobacteria Allergen according to the planed tuberculin skin tests in cattle of the farm must be implement.

3. In case of detection of tuberculin-positive test in animals the schedule diagnostic study of biomaterial for tuberculosis, in particular pathoanatomical, histological, bacteriological and biological must be performed.

4. Additional measures to reduce the population of atypical mycobacteria in animal sheds, other farm units in order to reduce the number of reservoirs must be developed.

Further researches will be directed on observance of regularity of preventive anti-tuberculosis measures, studying of epizootic features of mycobacteriosis manifestation caused by atypical Mycobacteria, pointing of sources and reservoirs of microorganisms, mechanism of their transfer from infective animals.

Keywords: tuberculosis, cattle, tuberculin, antigen of atypical mycobacteria, infectious process, atypical mycobacteria, allergic reactions, diagnosis.

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