Bulletin "Veterinary biotechnology"

Veterynarna biotehnologija – Veterinary biotechnology, 2020, 36, 21-33 [in Ukrainian]. https://doi.org/10.31073/vet_biotech36-02

GARKAVENKO T.A., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., GORBATIUK O.I., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., KOZITSKA T.G., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., ANDRIYASHCHUK V.O., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., DIBKOVA S.M., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

State Scientific and Research Institute of Laboratory Diagnostics and Veterinary and Sanitary Expertise

DETERMINATION OF VANCOMICIN-RESISTANT STRAINS (VRE) WITH VanA AND VanB ENZYMES AMONG FIELD ISOLATES OF Enterococcus spp., OBTAINED FROM DRINKING WATER SAMPLES

Introduction. The role of enterococci in current infectious pathology of animals and humans has increased dramatically. It has been proven that antibiotic resistant enterococci transmitting to humans through close contact with animals, via contaminated raw materials and products from the animal and poultry industries and environmental objects, including drinking water. The relevance of the study of Enterococcus spp. level of resistance to antimicrobials (AMP) is apparent because of the potential risks of infecting animals and humans with such pathogens.

The goal of the work was to determine the spectrum of antibiotic sensitivity of clinical isolates of Enterococcus spp., to identify among them VRE-strains and to confirm the production of VanA- and VanB-enzymes.

Materials and methods. 14 field isolates of Enterococcus spp. isolated from drinking water samples in the Odessa region were investigated. Sensitivity determination of experimental clinical isolates of Enterococcus spp. was performed by a disc-diffusion method using antibiotic disks according to EUCAST recommendations.

Results of research and discussion. Analysis of the results of studies to determine the sensitivity of Enterococcus spp. to AMP showed that 10 strains, from the studied 14, had sensitivity to all used antibiotics, that was 71,5% of all enterococcal isolates. Polyantibiotic resistance was established in 1 strain because it was insensitive to imipenem, levofloxacin, teicoplanin, and vancomycin. Among 4 strains belonging to the VRE were identified. Among them, 2 strains confirmed the production of VanA-enzymes and in 2 strains the production of VanB-enzymes.

Conclusions and prospects for further research.

1. Among the 14 clinical isolates of Enterococcus spp. 4 VRE strains were detected, which accounted for 28.5% of the studied.

2. Among the VRE strains of Enterococcus spp. 2 VanA-producing strains and 2 VanB-producing strains, 1 strain was characterized by polyresistance.

3. Frequency of selection of VRE strains of Enterococcus spp. from environmental sites confirms the necessity of obligatory bacteriological monitoring of zoonotic pathogens, determination of their sensitivity to AMP and identification of acquired mechanisms of resistance.

The prospect of further research is to study the level of resistance to AMP at film-forming strains of Enterococcus spp.

Keywords: Enterococcus spp., antibioticresistance, VRE-strains of Enterococcus spp., VanA-producing strains of Enterococcus spp., VanB-producing Enterococcus spp.

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