Veterynarna biotehnologija – Veterinary biotechnology, 2020, 36, 69-76 [in Ukrainian]. https://doi.org/10.31073/vet_biotech36-06
Institute of Veterinary Medicine NAAS
*State Scientific and Research Institute of Laboratory Diagnostics and Veterinary and Sanitary Expertise
TOTAL IMMUNOGLOBULINS MEASUREMENT IN BLOOD SERUM OF RABBITS VACCINATED WITH VACCINES CONTAINING METAL NANOPARTICLES
Introduction. Mixed infections play an important role in the etiology of infectious diseases. That is why it is necessary to create mixed vaccines that ensuring that animals have immune resistance to several diseases at the same time. Therefore, an urgent issue is the improvement of existing and the development of new vaccines for specific prevention of infectious diseases, especially those that have an associative course.
The goal of the work was to study total immunoglobulins indices in the serum of rabbits vaccinated with the vaccines "Velshicolisan + AuNP", "Velshicolisan + AgNP", "Velshicolysan".
Materials and methods. Experimental studies were conducted in the V.P. Ryzhenko laboratory of anaerobic infections of the Institute of veterinary medicine of the NAAS, vivarium of the V.P. Komisarenko Institute of endocrinology and metabolism of the NAMS and department of colloidal technology of natural system of the F.D. Ovcharenko Institute of biocolloid chemistry of the NAS.
Blood sampling for studies was performed before vaccination and in 7, 14, and 21 days after revaccination. Vaccination of animals was carried out in accordance with temporary guidelines for the use of experimental vaccines "Velshicolisan + AuNP", "Velshicolisan + AgNP", "Velshicolisan" against animals toxicosis caused by Clostridium perfringens and E. coli in the doses prescribed in these guidelines. Quantitative determination of total immunoglobulins of the serum of rabbits was performed according to generally accepted methods using 18% sodium sulfite solution.
Results of research and discussion. Analysis of the results of studies of the content of total immunoglobulins in the experimental and control groups of animals showed that at the beginning of the experiment it ranged from 9.0 mg/ml to 10.63 mg/ml; after 14 days of the experiment it increased by 3.9% and on the 7th day after revaccination (21 days from the beginning of the experiment) an increase of the indicator by 68.9% observed. The highest level of immunoglobulins observed on the 14 day after reimmunization (28 from the beginning of the experiment), this indicator was 18.4 mg/ml (78.6%), which increased by 1.7 times against the initial data and by 1.9 times against indicator of control animals. Results of the research of the serum of rabbits vaccinated with the vaccine "Velshicolisan + AgNP" (experimental group No. 2) showed that at the beginning of the experiment, the indicator of total immunoglobulin content was 10.63 mg/ml. After the second vaccination (7 days from the beginning of the experiment) this indicator increased by 11.95% to 11.85 mg/ml, compared to the initial data and was 11.2% higher than in animals of the control group. In the third group of experimental animals the total immunoglobulins indicator increased from day 7 after the beginning of the experiment to day 28. This indicates the positive effect of the vaccine on the synthesis of immunoglobulins.
Conclusion and prospect for future research:
1. The results of experimental studies confirm the effectiveness of using metal nanoparticles in biotechnology for vaccine manufacturing.
2. Experimental sample of the "Velshikolisan + AuNP" vaccine was the most effective, after 14 days after the second vaccination a significant increase of total immunoglobulins level in the blood serum of experimental rabbits was observed.
3. Double vaccination of animals with the vaccine "Velshikolisan + AuNP" provided the growth of total immunoglobulins 1.12 times at the end of the experiment.
4. The research is the initial stage for a complex test of the impact of the mentioned immunobiological agents on the animal organism. In the future, it is planned to conduct research on the effect of experimental vaccines on various parts of the animal immune system.
Keywords: rabbits, vaccines, immunoglobulins, metal nanoparticles.