Bulletin "Veterinary biotechnology"

Veterynarna biotehnologija – Veterinary biotechnology, 2020, 36, 111-117 [in Russian]. https://doi.org/10.31073/vet_biotech36-11

KRASOCHKO P.А., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., KRASOCHKO I.A., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., KASHPAR L.N., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

Vitebsk State Academy of Veterinary Medicine, Republic of Belarus

BORISOVETS D.S., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

S.N. Vyshelessky Institute of Experimental Veterinary Medicine of the National Academy of Sciences of Belarus

SHASHKOVA Y.А., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

BelVitunipharm, Republic of Belarus

NYCHYK S.A., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., TARASOV O.А., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

Institute of Veterinary Medicine NAAS of Ukraine

SELECTION OF NUTRIENT MEDIA FOR CULTIVATION OF CONTINUOUS ANIMAL CELL USING MICROCARRIERS UNDER CONDITIONS OF INDUSTRIAL ENTERPRISES

Introduction. Selection of optimal nutrient media for the cultivation of continuous animal cells in microcarriers under industrial conditions will increase their concentration, optimize the proliferation process and quality.

The goal of the work was to conduct selection of growth and supportive media for cell culture MDBK, SPEV, PT and VNK-21 using microcarriers based on modified polysaccharides under conditions of industrial enterprises.

Materials and methods. Research was conducted under JSC "Belvitunifarm" and UO "Vitebsk State Academy of Veterinary Medicine". For the study we used: 1) cell culture MDBK, PT, SPEV and VNK-21; 2) growth media: medium Eagle MEM, medium Eagle DMEM, medium 199, medium dry enzyme muscles (DEM) with Earle's medium, medium lactalbumin hydrolysate (LH) with Earle's medium; 3) сotton microcrystalline cellulose was used as a microcarrier based on modified polysaccharides. The cell concentration after cultivation was counted in hemocytometer under the small magnification of the microscope.

Results of a research and discussion. The results of determining the cells concentration after MDBK, PT and SPEV cells cultures cultivation showed that their greatest concentration is achieved by cultivation in medium including Eagle DMEM + 199 (1:1). However for VNK-21 cells cultivation the optimal was medium DEM with Earle's medium, medium LH with Earle's medium. Synthetic media for the cultivation of these cells are less active.

Conclusions and prospects for further researches. The data obtained indicate the feasibility of using media Eagle DMEM +199 (1:1). For MDBK, PT and SPEV cell cultures and medium DEM with Earle's medium, medium LH with Earle's medium for VNK-21 cell culture using microcarriers based on modified polysaccharides in industrial plants.

Keywords: microcarriers, modified polysaccharides, cells culture, growth medium.

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