Bulletin "Veterinary biotechnology"

Veterynarna biotehnologija – Veterinary biotechnology, 2020, 36, 118-128 [in Ukrainian]. https://doi.org/10.31073/vet_biotech36-12

KRYVOSHYIA P., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

Research Station Of Epizootology IVM NAAS

RUD О., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

Rivne State University of Humanitie


Introduction. According to the current classification, all isolated strains of equine influenza virus are classified into type A, belonging to two different subtypes: H7N7 (1st) and H3N8 (2nd). The greatest damage is caused by the disease in the period of epizootics occurring with a certain frequency. Vaccination is an effective way of prevention, but the success of it depends largely on the immunobiological condition of the animal.

The goal of the work. Investigate the vaccination intensity of influenza vaccine immunity and its connection with the immunobiological condition and age of horses.

Materials and methods. Albumin content, globulin fractions, immune complexes, immunoglobulins, homogemagglutinins, heterohemagglutinins, erythrocyte counts and hematocrit were conducted according to conventional methods. Determination of specific antibodies to equine influenza virus was performed using the serological method – hemagglutination inhibition test (HNA). HNA included the following main steps: determination of the haemagglutinating titer (1 GAO) of the virus, selection of the working dose (4 GAO), and execution of the method with determination of the antibody content.

Results of research and discussion. 120 serums of blood were investigated before the vaccination with an influenza vaccine in different periods. The titer of antibodies before the vaccination was 1:20 to 1:40 and through a month – rose up to 1:160 – 1:1280, through five months it was identified and amounted 1:20 – 1:160. The features of immune answer for an influenza vaccine in the mixed aged groups of horse were defined. Established that among the vaccinated horses with the increase of concentration of specific antibodies to the antigens of influenza virus vaccine strains the content of albumin, β-globulins, erythrocyte, hematocrit decrease and the level of immune complexes, content of γ-globulins, title of heterohemagglutinins and homohemagglutinins increase. It was confirmed that horses vaccinated by the vaccine "Fluekvin" acquired high tension of immunity during five months after vaccinations.

Conclusions and prospects for further research. Installed, that horses vaccinated with "Fluekvin" have been shown to maintain high levels of immunity for five months after vaccination. A more active and long-lasting immune response was observed in the age group of horses from 3 to 9 years after vaccination than in horses aged 10 to 19 years.

In further studies, it is advisable to study the functional and quantitative status of immunocomponent cells in the post-vaccinated period in vaccinated horses with the influenza vaccine.

Keywords: horses, influenza vaccine, age, post-vaccination period, immunobiological indexes, anti hemagglutinins.


  1. Krivoshyia, P.Yu. (2013). Epizootolohichnyy monitorynh infektsiynykh zakhvoryuvan koney ta shlyakhy pidvyshchennya yoho efektyvnosti [Epizootological monitoring of infectious diseases of horses and ways of increasing its efficiency]. Veterynarna medytsyna Ukrayiny – Veterinary medicine of Ukraine, 3, 7-10 [in Ukrainian].
  2. Siuryn, V.N., Belousova, R.V., & Fomina, N.V. (1991). Veterinarnaya virusologiya [Veterinary Virology]. M: Agropromizdat [in Russian].
  3. Siuryn, V.N., Belousova, R.V., & Fomina, N.V. (1991). Diagnostika virusnykh bolezney zhivotnykh: Spravochnik [Diagnosis of viral diseases of animals: Guide]. M: Agropromizdat [in Russian].
  4. Starcheus, A.P., & Obrazhei, A.F. (1999). Virusni khvoroby koney [Viral diseases of horses]. Kyiv [In Ukrainian].
  5. Gusakov, V.V. (1998). Epizootychna sytuatsiya shchodo hrypu koney na Kyyivskomu ipodromi v 1995–1996 rr. [The epizootic situation of equine influenza on the Kiev racetrack in 1995–1996]. Veterynarna medytsyna Ukrayiny – Veterinary medicine of Ukraine, 10, 32-33 [in Ukrainian].
  6. Krivoshyia, P.Yu. (2018). Imunobiokhimichnyy stan koney Zakhidnoho Polissya Ukrayiny [Immunobiochemical condition of horses of the Western Polesie of Ukraine]. Hamburg: LAP LAMBERT Academic Publishing [in Ukrainian].
  7. Krivoshyia, P.Yu., Kot, L.B., & Romanko, M.V. (2014). Identyfikatsiya izolyatu virusu hrypu koney Zakhidnoho Polissya Ukrayiny [Identification of a horse flu virus isolate of Western Polesie of Ukraine]. VIII Vseukr. nauk.-prakt. konf. molodykh vchenykh (October 16-17, 2014) – VIII All-Ukrainian Research Practice Conf. of Young Scientists. (pp. 35-37). Kharkiv [in Ukrainian].
  8. Busol, V.A., Mandygra, M.S., Galatiuk, O.E., Krivoshyia, P.Yu., & Samsoniuk, L.S. (1996). Metodychni rekomendatsiyi po otsintsi imunnoho statusu koney v normi ta za prykhovanoho perebihu infektsiynoyi anemiyi [Methodical recommendations for the evaluation of the immune status of horses in the normal state and in the hidden course of infectious anemia]. Rivne [in Ukrainian].

Download full text in PDF