Bulletin "Veterinary biotechnology"

Veterynarna biotehnologija – Veterinary biotechnology, 2020, 36, 128-137 [in Ukrainian]. https://doi.org/10.31073/vet_biotech36-13

NECHYPORENKO O., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., BEREZOVSKIY A., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., SHKROMADA O., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., ULKO L., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

Sumy National Agrarian University

NYCHYK S.A., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

Institute of Veterinary Medicine NAAS

ASSESSMENT OF ADG DISINFECTANT CUMULATIVE AND METABOLIC RATE EFFECT IN LABORATORY ANIMALS

Introduction. The ADG disinfectant contains glutaraldehyde, benzalkonium chloride, dodecyldimethylammonium chloride, isopropyl alcohol in certain proportions. Preventive disinfection is performed in the poultry farms, therefore it is necessary to determine the safety of the disinfectant to the macroorganism.

The goal of the work. Determination of ADG disinfectant cumulative and metabolism rate effect on the organism of laboratory animals.

Materials and methods. White rats were used for toxicity determination. Animals of three-month age weighing 180-200 g (Wistar line) were selected in two groups experimental and control of 15 rats each. Studies of the level of cumulative toxicity were performed using "Subchronic Toxicity" by K.S. Lim and co-authors method in K.K. Sidorov modification. The experimental group received ADG disinfectant orally by 380 mg/kg of body weight of the rat or 1/10 DL50. Every 4 days the dose was gradually increased by 1.5 times. During the experiment, the clinical condition and death of experimental animals were monitored.

Results of research and discussion. It was experimentally determined that the LD50 of ADG disinfectant per rat used for 20 days was 20040 mg/kg. It is proved that the ADG disinfectant has a weak cumulative effect with a cumulation coefficient of 5.27 units. The tests on white rats showed that solution of disinfectant ADG demonstrates weak cumulative peculiarities. On the 20th day the animals had an appetite, normal functional state of the central nervous system. The shape of liver was enlargement with tension of the capsule, the color was uneven.

Conclusions and prospects for further research. ADG disinfectant caused toxic effects on animals in high doses, which was manifested by a significant decrease in body weight of laboratory animals within 20 days; significant increase in the coefficients of mass of hematopoietic organs, such as liver – by 23.8%, spleen – by 15.2% and mass of kidneys by 30.1% (p <0.001), compared with similar indicators in control group. The ADG side effects to organism of laboratory animals is under our study.

Keywords: ADG disinfectant, internal organ mass index, toxicity, animal body weight.

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